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Distribution patterns of calcium-binding proteins in pancreatic tissue of non-diabetic as well as type 2 diabetic rats and in rat insulinoma β-cells (INS-1)

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Abstract

The present study dealt with the localization of different calcium-binding proteins (CaBPs) in the pancreatic tissue of non-diabetic and diabetic rats and in rat insulinoma β-cells (INS-1). Transcripts of CaBPs displayed different expression levels in rat pancreatic tissue and INS-1 cells. Immunohistochemistry demonstrated that three of these proteins, calmodulin, calreticulin and calbindin-D28k, were located predominantly in the pancreatic islets (in both α- and β-cells) of rats, showing weaker labeling of exocrine tissue. Secretagogin was exclusively found within islets. All CaBPs were also immunohistochemically detected in INS-1 cells. Immunohistochemical analysis demonstrates differences in CaBP distributions when comparing the pancreatic tissues of diabetic Goto-Kakizaki rats and non-diabetic Wistar rats. Pancreatic tissue in type 2 diabetic Goto-Kakizaki rats showed significantly higher transcript levels of all CaBPs compared to those in Wistar rats. These results indicate that alterations of CaBPs in pancreatic islets are associated with metabolic disturbances related to type 2 diabetes.

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Acknowledgments

We wish to thank Prof. C. Wollheim from the Department of Medicine, University Geneva, Switzerland, for making the INS-1 cell line available to us. The authors also thank Dr. L. Litvak for technical assistance and Ms. Mühlbauer for linguistic assistance. This study was supported by a grant from ROUX-Anschubförderung FKZ 15/13, Martin Luther University, Halle-Wittenberg.

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  1. Institute of Anatomy and Cell Biology, Martin Luther University Halle-Wittenberg, Grosse Steinstrasse 52, 06097, Halle, Germany

    Ivonne Bazwinsky-Wutschke & Elmar Peschke

  2. Saxon Academy of Sciences Leipzig, Karl-Tauchnitz-Strasse 1, 04107, Leipzig, Germany

    Sabine Wolgast & Eckhard Mühlbauer

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  1. Ivonne Bazwinsky-Wutschke
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Bazwinsky-Wutschke, I., Wolgast, S., Mühlbauer, E. et al. Distribution patterns of calcium-binding proteins in pancreatic tissue of non-diabetic as well as type 2 diabetic rats and in rat insulinoma β-cells (INS-1). Histochem Cell Biol 134, 115–127 (2010). https://doi.org/10.1007/s00418-010-0721-y

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