Abstract
Azelaic acid (AzA, 1,9-nonadienoic acid) is a nine-carbon chain (C9) dicarboxylic acid with multiple and diverse functions in humans and plants. In plants this compound was suggested as a marker for lipid peroxidation under biotic and abiotic stress conditions and an inducer (priming agent) of plant immunity (acquired resistance). Detection methods for AzA in plants include a wide range of methodological approaches. This new and simple reversed-phase HPLC-MS protocol describes the measurement of AzA and other dicarboxylic acids either from tobacco leaf tissue or petiolar exudates (vascular sap) of plants under non-derivatized conditions.
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Acknowledgments
The authors wish to thank the Hungarian National Research, Development and Innovation Office for funding (ALÁ, K112146; LK, K111995 and K128868; AK, FK131401).
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Ádám, A.L., Kátay, G., Künstler, A., Király, L. (2022). Detection of Lipid Peroxidation-Derived Free Azelaic Acid, a Biotic Stress Marker and Other Dicarboxylic Acids in Tobacco by Reversed-Phase HPLC-MS Under Non-derivatized Conditions. In: Mhamdi, A. (eds) Reactive Oxygen Species in Plants. Methods in Molecular Biology, vol 2526. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2469-2_14
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DOI: https://doi.org/10.1007/978-1-0716-2469-2_14
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