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Molecular Imaging of Tumor Progression and Angiogenesis by Dual Bioluminescence

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Bioluminescence

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2524))

Abstract

Angiogenesis is a prerequisite for tumor growth and invasion, and anti-angiogenesis has become a highlight in tumor treatment research. However, so far, there is no reliable solution for how to simultaneously visualize the relationship between tumor progression and angiogenesis. Bioluminescence imaging (BLI) has been broadly utilized and is a very promising non-invasive imaging technique with the advantages of low cost, high sensitivity, and robust specificity. In this chapter, we describe a dual bioluminescence imaging BLI protocol for tumor progression and angiogenesis through implanting murine breast cancer cell line 4T1 which stably expressing Renilla luciferase (RLuc) into the transgenic mice with angiogenesis-induced firefly luciferase (FLuc) expression. This modality enables us to synchronously monitor the tumor progression and angiogenesis in the same mouse, which has broad applicability in oncology studies.

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Acknowledgments

This research was supported by the National Key R&D Program of China (2017YFA0103200), National Natural Science Foundation of China-Henan Joint Fund (U2004126), and Tianjin Natural Science Foundation (21JCZDJC00070).

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Authors and Affiliations

  1. School of Medicine, Nankai University, Tianjin, China

    Yue Liu, Ziyu Huang & Zongjin Li

  2. The Key Laboratory of Bioactive Materials, The College of Life Science, Ministry of Education, Nankai University, Tianjin, China

    Yue Liu, Ziyu Huang & Zongjin Li

  3. Tianjin Key Laboratory of Human Development and Reproductive Regulation, Nankai University Affiliated Hospital of Obstetrics and Gynecology, Tianjin, China

    Zongjin Li

  4. State Key Laboratory of Kidney Diseases, Chinese PLA General Hospital, Beijing, China

    Zongjin Li

Authors
  1. Yue Liu
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  2. Ziyu Huang
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Corresponding author

Correspondence to Zongjin Li .

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Editors and Affiliations

  1. Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan

    Sung-Bae Kim

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Liu, Y., Huang, Z., Li, Z. (2022). Molecular Imaging of Tumor Progression and Angiogenesis by Dual Bioluminescence. In: Kim, SB. (eds) Bioluminescence. Methods in Molecular Biology, vol 2524. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2453-1_34

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  • DOI: https://doi.org/10.1007/978-1-0716-2453-1_34

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2452-4

  • Online ISBN: 978-1-0716-2453-1

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