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Bioluminescent and Fluorescent Reporter-Expressing Recombinant SARS-CoV-2

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Bioluminescence

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2524))

Abstract

Reporter-expressing recombinant severe acute respiratory syndrome coronavirus 2 (rSARS-CoV-2) represents an excellent tool to understand the biology of and ease studying viral infections in vitro and in vivo. The broad range of applications of reporter-expressing recombinant viruses is due to the facilitated expression of fluorescence or bioluminescence readouts. In this chapter, we describe a detailed protocol on the generation of rSARS-CoV-2 expressing Venus, mCherry, and NLuc that represents a valid surrogate to track viral infections.

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Acknowledgments

We would like to thank the members at Texas Biomedical Research Institute for their efforts in keeping them fully operational during the COVID-19 pandemic and the Institutional Biosafety Committee at Texas Biomedical Research Institute for reviewing our protocols in a time-efficient manner. SARS-CoV-2 research in the Martinez-Sobrido’s laboratory is currently supported by the NIAID/NIH grants RO1AI161363-01, RO1AI161175-01A1, and R43AI165089-01; the Department of Defense (DoD) grants W81XWH2110095 and W81XWH2110103; the San Antonio Partnership for Precision Therapeutic; the Texas Biomedical Research Institute Forum; the University of Texas Health Science Center at San Antonio; the San Antonio Medical Foundation; and the Center for Research on Influenza Pathogenesis and Transmission (CRIPT), a NIAID-funded Center of Excellence for Influenza Research and Response (CEIRR, contract # 75N93021C00014).

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Authors and Affiliations

  1. Texas Biomedical Research Institute, San Antonio, TX, USA

    Desarey Morales Vasquez, Kevin Chiem, Chengjin Ye & Luis Martinez-Sobrido

  2. Department of Disease Prevention and Intervention, Texas Biomedical Research Institute, San Antonio, TX, USA

    Luis Martinez-Sobrido

Authors
  1. Desarey Morales Vasquez
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  2. Kevin Chiem
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  3. Chengjin Ye
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  4. Luis Martinez-Sobrido
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Corresponding author

Correspondence to Luis Martinez-Sobrido .

Editor information

Editors and Affiliations

  1. Environmental Management Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki, Japan

    Sung-Bae Kim

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Morales Vasquez, D., Chiem, K., Ye, C., Martinez-Sobrido, L. (2022). Bioluminescent and Fluorescent Reporter-Expressing Recombinant SARS-CoV-2. In: Kim, SB. (eds) Bioluminescence. Methods in Molecular Biology, vol 2524. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2453-1_18

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  • DOI: https://doi.org/10.1007/978-1-0716-2453-1_18

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2452-4

  • Online ISBN: 978-1-0716-2453-1

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