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Evaluation of Pharmaceutical Inhibition of Vasculogenic Mimicry In Vitro

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Vasculogenic Mimicry

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2514))

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Abstract

Vasculogenic mimicry formation is generally assessed using three-dimensional (3D) cultures of aggressive tumor cells grown over an extended incubation period. Test agents can be introduced during growth of the 3D cultures to determine their effect on vasculogenic mimicry formation. Here, we describe the protocol for evaluation of the inhibitory effect of drugs on vasculogenic mimicry in vitro using bright-field and fluorescence microscopy on 3D cultures of tumor cells grown in Matrigel.

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Acknowledgments

This work was supported by NIH grant P30 EY007551, RO1 EY017120, Texas ARP, and 2011 UHCO Student Vision Science Grants to advance Research to J.C.M. MUM2b cells were generously provided by Dr. Arthur S. Polans from the Department of Ophthalmology and Vision Science of the University of Wisconsin. OCM 8 cells, originally developed by Dr. June Kan-Mitchell from the University of California, were generously provided by Dr. Jerry Niederkorn, University of Texas Southwestern Dallas.

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Authors and Affiliations

  1. College of Optometry, University of Houston, Houston, TX, USA

    Joseph C. Manarang & Alison McDermott

Authors
  1. Joseph C. Manarang
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  2. Alison McDermott
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Editor information

Editors and Affiliations

  1. InteLab/EQA, Federal Institute of Santa Catarina, Florianopolis, Brazil

    Emily Marques dos Reis

  2. Human Cell and Tissue Engineering, Biocelltis Bioengineering, Florianopolis, Brazil

    Fernanda Berti

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Manarang, J.C., McDermott, A. (2022). Evaluation of Pharmaceutical Inhibition of Vasculogenic Mimicry In Vitro. In: Marques dos Reis, E., Berti, F. (eds) Vasculogenic Mimicry. Methods in Molecular Biology, vol 2514. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2403-6_13

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  • DOI: https://doi.org/10.1007/978-1-0716-2403-6_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2402-9

  • Online ISBN: 978-1-0716-2403-6

  • eBook Packages: Springer Protocols

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