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Generation of Plasmodium falciparum Gametocytes In Vitro with Specific Considerations for Field Isolates

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Malaria Immunology

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2470))

Abstract

Reproducible induction of gametocytes of Plasmodium falciparum in vitro is crucial for performing various experimental analyses to understand gametocyte cellular and molecular biology and immunology, and for the evaluation of antigametocidal agents and vaccine development. In this protocol, we present specific procedures for the enrichment, synchronous production and separation of developmental stages of P. falciparum gametocytes from culture-adapted field isolates.

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Acknowledgments

We thank Maha Saeed for initial training and helpful discussions. This work was supported by a UK Wellcome Trust grant (110090/Z/15/Z) to B.D and C.S.

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Authors and Affiliations

  1. Department of Biomedical Sciences, School of Basic and Biomedical Sciences, University of Health and Allied Sciences, Ho, Ghana

    Bismarck Dinko & Richmond Tackie

  2. Department of Infection Biology, Faculty of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London, UK

    Gisela Henriques, Lindsay Stewart & Colin Sutherland

Authors
  1. Bismarck Dinko
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  2. Richmond Tackie
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  3. Gisela Henriques
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  4. Lindsay Stewart
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  5. Colin Sutherland
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Corresponding author

Correspondence to Bismarck Dinko .

Editor information

Editors and Affiliations

  1. Centre for Medical Parasitology, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark

    Anja Tatiana Ramstedt Jensen

  2. Centre for Medical Parasitology, Department of Immunology and Microbiology, University of Copenhagen, Copenhagen, Denmark

    Lars Hviid

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Dinko, B., Tackie, R., Henriques, G., Stewart, L., Sutherland, C. (2022). Generation of Plasmodium falciparum Gametocytes In Vitro with Specific Considerations for Field Isolates. In: Jensen, A.T.R., Hviid, L. (eds) Malaria Immunology. Methods in Molecular Biology, vol 2470. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2189-9_11

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  • DOI: https://doi.org/10.1007/978-1-0716-2189-9_11

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-2188-2

  • Online ISBN: 978-1-0716-2189-9

  • eBook Packages: Springer Protocols

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