Abstract
Galectin-1 (gal-1), a member of a family of evolutionarily conserved glycan-binding proteins, is differentially expressed at the feto–maternal interface and appears to be functionally polyvalent, with a wide range of biological activities. However, the contributions of maternal and/or feto-placental gal-1 to the signaling networks promoting a healthy pregnancy are still being elucidated. This chapter discusses the methods commonly employed to study the maternal or feto-placental contribution of gal-1 during pregnancy in mice. The methods described here can be used to decipher the specific role of each source, e.g., maternal and/or feto-placental derived gal-1 in the orchestration of pregnancy-associated processes.
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Acknowledgments
Funding Source: Methods discussed in this chapter were part of the research supported by the Deutsche Forschungsgemeinschaft (BL1115/2-1, BL1115/4-1 and Heisenberg program BL1115/7-1) to S.M.B. and Else Kröner-Fresenius-Stiftung (2017_A123) to N.F.
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Borowski, S., Freitag, N., Urban, I., Michel, G., Barrientos, G., Blois, S.M. (2022). Examination of the Contributions of Maternal/Placental-Derived Galectin-1 to Pregnancy Outcome. In: Stowell, S.R., Arthur, C.M., Cummings, R.D. (eds) Galectins. Methods in Molecular Biology, vol 2442. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2055-7_32
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DOI: https://doi.org/10.1007/978-1-0716-2055-7_32
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