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Placental Expression and Relative Role of Anti-inflammatory Annexin A1 and Animal Lectin Galectin-3 in the Pathogenesis of Preeclampsia

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Abstract

Preeclampsia (PE) remains the major cause for maternal and foetal mortality and morbidity all over the world. Preeclampsia is associated with maternal, placental aggravated inflammatory response and generalized endothelial damage. AnnexinA1 (AnxA1) is glucocorticoid regulated protein regulates a wide range of cellular and molecular steps of the inflammatory response and is implicated in resolution of inflammation. Galectin-3 (Gal-3), β-galcotoside-binding lectin participates in many functions, both intra- and extracellularly. Recently it has been shown that galectin-3 modulates the inflammation. Role of AnxA1 and Galectin-3 is poorly studied in context with human reproductive disease like Preeclampsia. Therefore, the present study examined the expression of AnxA1 and Gal-3 which are involved in modulation of inflammation and their association in the placental bed of pregnancy with and without PE. The study group consisted of placental bed biopsy tissues obtained from pregnancies with PE (n = 30) and without (n = 30) PE. The expression of AnxA1 and Gal-3 in the placental bed tissues was evaluated quantitatively using Immunohisto-chemistry (IHC), western blot and mRNA expression analysis by quantitative RT-PCR. Our IHC, western blot and RT PCR analyses showed the increase in the expression of AnxA1 and Gal-3 in PE group compared with the normotensive control group (P < 0.001). The increased expression of AnxA1 and Gal-3 in placental bed may be associated with a systemic inflammatory response in PE, suggesting role of AnxA1 and Gal-3 in PE pathogenesis.

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Acknowledgments

The authors would like to acknowledge Mr Aslam for tissue processing and Mr. Gururaj for the technical help.

Funding

The authors received no financial support for the research.

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Authors and Affiliations

  1. Department of Physiology, Shri B. M. Patil Medical College, Hospital & Research Centre, BLDE (Deemed To Be University), Vijaypur, India

    Komal Ruikar & Manjunath Aithal

  2. Department of Biochemistry, K. S. Hegde Medical Academy, Nitte (Deemed To Be University), Mangalore, India

    Praveenkumar Shetty

  3. Central Research Lab, K. S. Hegde Medical Academy, Nitte (Deemed to be University), Mangalore, India

    Praveenkumar Shetty, E. Sarathkumar & Prakash Patil

  4. Department of Pathology, SDM College of Medical Sciences and Hospital, Dharwad, India

    Udupi Shastry Dinesh

  5. Department of Biochemistry, SDM College of Medical Sciences and Hospital, Dharwad, India

    Anil Bargale

  6. Department of Anatomy, SDM College of Medical Sciences and Hospital, Dharwad, India

    Roshni Sadashiv

  7. Department of Physiology, SDM College of Medical Sciences and Hospital, Dharwad, India

    Komal Ruikar & Vitthal Khode

  8. Department of Obstetrics and Gynaecology, SDM College of Medical Sciences and Hospital, Dharwad, India

    Rathnamala Desai

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  1. Komal Ruikar
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Contributions

Conceived the idea and designed the experiments: PKS and KR; Helped in clinical sample collection and correlated the clinical relevance to the study: KR, PKS, MA and VK; Performed the experiments: KR, SE, AB, and RS; Analysed the data: USD, KR, PKS, PP, AB and VK; Manuscript preparation: KR, PKS and PP; Supervised the overall study: PKS and MA.

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Correspondence to Praveenkumar Shetty.

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The author declare that they have no conflict of interest.

Ethical Approval

All procedures performed in studies involving human participants were in accordance with the ethical standards of the institutional and national research committee and with the 1964 Helsinki declaration and its later amendments or comparable ethical standards. The study was approved by the Ethical Committee of SDM College of Medical Sciences and Hospital, Dharwad, Karnataka, India (SDM IEC: 0748: 2016).

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Informed consent was obtained from all individual participants included in the study.

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Ruikar, K., Aithal, M., Shetty, P. et al. Placental Expression and Relative Role of Anti-inflammatory Annexin A1 and Animal Lectin Galectin-3 in the Pathogenesis of Preeclampsia. Ind J Clin Biochem 37, 60–68 (2022). https://doi.org/10.1007/s12291-020-00952-z

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  • DOI: https://doi.org/10.1007/s12291-020-00952-z

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