Abstract
Mass spectrometry imaging (MSI) allows label-free detection of a wide range of biomolecules and simultaneously provides their spatial distributions. In particular, MSI by matrix-assisted laser desorption/ionization mass spectrometry (MALDI) has been widely used in biomolecule analysis. However, quantitation in MALDI-MSI is limited by matrix-deposition heterogeneity, analyte extraction area, and analyte–matrix cocrystallization. In this chapter, a microstructured PDMS stamp is utilized to precisely control the matrix deposition area and the analyte extraction area. We describe here simple steps—including stamp fabrication, matrix application, and data-acquisition guideline—for the quantitative analysis of adsorbed peptides on hydrophobic surfaces.
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Acknowledgments
This work was supported by the Basic Science Research Program through the National Research Foundation (NRF) of Korea (NRF-2019R1A2C1084928).
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Park, S., Lee, C.Y. (2022). Extraction and Upconcentration of Adsorbates from Precisely Defined Area for Quantitative MALDI Mass Spectrometry Imaging. In: Lee, YJ. (eds) Mass Spectrometry Imaging of Small Molecules. Methods in Molecular Biology, vol 2437. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-2030-4_11
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DOI: https://doi.org/10.1007/978-1-0716-2030-4_11
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Publisher Name: Humana, New York, NY
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