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Isolation of Normal and Cancer-Associated Fibroblasts

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Ovarian Cancer

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2424))

Abstract

Cancer-associated fibroblasts (CAFs) play important roles in regulating tumor progression, metastasis, and response to therapies. Accurately modeling the interplay between cancer cells and the tumor microenvironment (TME) requires the use of primary cells from patient samples. Here we describe methods for the isolation of both primary CAFs and fibroblasts from omental tissue using a combination of mechanical dissociation and enzymatic digestion. Primary cells can be used for functional and mechanistic studies and may be safely cryopreserved.

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Acknowledgments

We would like to thank all of the members of the Lengyel Ovarian Cancer Research Laboratory at the University of Chicago for their helpful comments and feedback. In particular, we thank Dr. Abir Mukherjee for his assistance. M.A.E. was supported by the Ovarian Cancer Research Alliance (OCRA) Liz Tilberis Early Career Award 650339.

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Authors and Affiliations

  1. Department of Obstetrics and Gynecology/Section of Gynecologic Oncology, University of Chicago, Chicago, IL, USA

    Katarzyna Zawieracz & Mark A. Eckert

Authors
  1. Katarzyna Zawieracz
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  2. Mark A. Eckert
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Correspondence to Mark A. Eckert .

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Editors and Affiliations

  1. Department of Biomedical Engineering, University of Wisconsin-Madison, Madison, WI, USA

    Pamela K. Kreeger

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© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature

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Zawieracz, K., Eckert, M.A. (2022). Isolation of Normal and Cancer-Associated Fibroblasts. In: Kreeger, P.K. (eds) Ovarian Cancer. Methods in Molecular Biology, vol 2424. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1956-8_10

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  • DOI: https://doi.org/10.1007/978-1-0716-1956-8_10

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1955-1

  • Online ISBN: 978-1-0716-1956-8

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