Abstract
Protozoan pathogen contamination of leafy green presents a health risk for fresh produce consumers. This chapter describes a simple multiplex PCR (mPCR ) assay for simultaneous detection of Cryptosporidium spp., Giardia spp., Cyclospora cayetanensis, and Toxoplasma gondii on leafy greens . The nested mPCR assay provides a rapid, inexpensive, and simple approach for simultaneous detection of protozoan pathogens on fresh produce .
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Acknowledgments
Development of the mPCR assay was supported by the Center for Produce Safety and the California Department of Food and Agriculture.
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Kim, M., Shapiro, K. (2021). Detection of Protozoan Parasites on Leafy Greens Using Multiplex PCR. In: Magnani, M. (eds) Detection and Enumeration of Bacteria, Yeast, Viruses, and Protozoan in Foods and Freshwater. Methods and Protocols in Food Science . Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1932-2_15
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DOI: https://doi.org/10.1007/978-1-0716-1932-2_15
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