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Real-Time Polymerase Chain Reaction (PCR) Based Identification and Detection of Fungi Belongs to Genus Fusarium

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Molecular Markers in Mycology

Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

The genus Fusarium is of great importance, since members of this genus are plant-pathogens as well as human-pathogens, and the secondary metabolites produced by Fusarium species are hazardous to agricultural products, wildlife, livestock and humans. The classical method of identification and detection based on morphological characteristics are time consuming and erroneous, which complicate timely disease management decisions. The real-time PCR based molecular techniques are more specific, sensitive and accurate than traditional methods. Early and reliable detection is crucial for implementation of disease control strategies, and for the disease management. These assays can function as a basis for clinical labs, regulatory personnel, and other diagnosticians to adapt or implement for detection of human, plant and animal pathogenic Fusarium species. In the present chapter, we present overview on the basic technique of real-time PCR for the accurate quantification, detection and diagnosis of plant and human pathogenic Fusarium species.

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Authors and Affiliations

  1. Department of Otorhinolaryngology-Head and Neck Surgery & Institute for Medical Device Clinical Trials, Korea University college of Medicine, Korea University, Seoul, South Korea

    Mukesh Kumar Yadav

  2. Molecular Microbiology and Systematic Laboratory, Department of Biotechnology, Aizawl, Mizoram University, Aizawl, Mizoram, 796004, India

    Bhim Pratap Singh

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  1. Mukesh Kumar Yadav
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Correspondence to Mukesh Kumar Yadav .

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Editors and Affiliations

  1. Department of Biotechnology, Mizoram University, Aizawl, Mizoram, India

    Bhim Pratap Singh

  2. Department of Biochemistry, National University of Ireland Galway, Galway, Ireland

    Vijai Kumar Gupta

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Yadav, M.K., Singh, B.P. (2017). Real-Time Polymerase Chain Reaction (PCR) Based Identification and Detection of Fungi Belongs to Genus Fusarium. In: Singh, B.P., Gupta, V.K. (eds) Molecular Markers in Mycology. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-319-34106-4_4

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