Abstract
The Bordetella pertussis bacterium is the causative agent of whooping cough (pertussis disease). Following recent outbreaks of pertussis, disproportionately affecting young infants, several countries have introduced maternal pertussis vaccination strategies, aimed at boosting transplacental transfer of protective antibodies during pregnancy. Given historical associations between high maternal antibody and blunted infant responses to vaccination, subsequent research studies have investigated the impact of maternal pertussis vaccine on infant humoral responses. However, far less is known about the potential impact of the vaccine on innate immunity. Here, we describe methods to detect in vitro cellular responses to B. pertussis in mothers and their infants using a B. pertussis stimulation assay and multiplex cytokine assays to address this research question.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Similar content being viewed by others
References
Sawyer M, Liang JL, Messonnier N, Clark TA (2012) Updated recommendations for use of tetanus toxoid, reduced diphtheria toxoid, and acellular pertussis vaccine (Tdap) in pregnant women--advisory committee on immunization practices (ACIP). Morb Mortal Wkly Rep 62(7):131–135
Wiley KE, Massey PD, Cooper SC et al (2013) Pregnant women’s intention to take up a post-partum pertussis vaccine, and their willingness to take up the vaccine while pregnant: a cross sectional survey. Vaccine 31(37):3972–3978
Donegan K, King B, Bryan P (2014) Safety of pertussis vaccination in pregnant women in UK: observational study. BMJ 349(9526):g4219
Gall SA, Myers J, Pichichero M (2011) Maternal immunization with tetanus–diphtheria–pertussis vaccine: effect on maternal and neonatal serum antibody levels. Am J Obstet Gynecol 204(4):334. e1
Palmeira P, Quinello C, Silveira-Lessa AL et al (2012) IgG placental transfer in healthy and pathological pregnancies. Clin Dev Immunol 2012:985646
Byrne P, McGuirk P, Todryk S, Mills KHG (2004) Depletion of NK cells results in disseminating lethal infection with Bordetella pertussis associated with a reduction of antigen-specific Th1 and enhancement of Th2, but not Tr1 cells. Eur J Immunol 34(9):2579–2588
Higgins SC, Lavelle EC, McCann C et al (2003) Toll-like receptor 4-mediated innate IL-10 activates antigen-specific regulatory T cells and confers resistance to Bordetella pertussis by inhibiting inflammatory pathology. J Immunol 171(6):3119–3127
Mahon BP, Sheahan BJ, Griffin F et al (1997) Atypical disease after Bordetella pertussis respiratory infection of mice with targeted disruptions of interferon-γ receptor or immunoglobulin μ chain genes. J Exp Med 186(11):1843–1851
Kroes MM, Mariman R, Hijdra D et al (2019) Activation of human NK cells by Bordetella pertussis requires inflammasome activation in macrophages. Front Immunol 10:2030
Lee AH, Shannon CP, Amenyogbe N et al (2019) Dynamic molecular changes during the first week of human life follow a robust developmental trajectory. Nat Commun 10(1):1–14
Thalen M, Van Den Ijssel J, Jiskoot W et al (1999) Rational medium design for Bordetella pertussis: basic metabolism. J Biotechnol 75(2–3):147–159
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2022 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Rice, T., Holder, B. (2022). Determination of Maternal and Infant Immune Responses to Pertussis Vaccination in Pregnancy. In: Bidmos, F., Bossé, J., Langford, P. (eds) Bacterial Vaccines. Methods in Molecular Biology, vol 2414. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1900-1_17
Download citation
DOI: https://doi.org/10.1007/978-1-0716-1900-1_17
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1899-8
Online ISBN: 978-1-0716-1900-1
eBook Packages: Springer Protocols