Abstract
Keratinases are enzymes of the protease class which have the ability to hydrolyse keratin, a structural protein of animal origin. These enzymes find applications in different areas such as agriculture, textiles, cosmetics, among others. Alike other enzymes, keratinases can be obtained from bioprocesses realized by microorganisms, among which, actinobacteria figure as good producers. The objective of this chapter is to provide an overview of recent studies on the production of keratinase by actinobacteria and present experimental protocols for the isolation and culture conditions of the actinobacteria, as well as the analysis of the enzyme activity. Studies found in the literature report different species of keratinase-producing actinobacteria, and the cultivation conditions vary widely as concerns pH, temperature, time, agitation, and other parameters. However, chicken feathers have been the most utilized substrate as a medium of the production of keratinase.
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Maldonado, R.R., Souza, T.R.C., Kubeneck, S., Aguiar-Oliveira, E., Treichel, H. (2022). Isolation and Cultivation of Actinobacteria by Submerged Fermentation for the Production of Keratinase. In: Dharumadurai, D. (eds) Methods in Actinobacteriology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1728-1_76
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DOI: https://doi.org/10.1007/978-1-0716-1728-1_76
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