Abstract
Strigolactones (SLs) have recently discovered an important group of plant hormones. Strigolactones are biosynthesized in roots followed by transportation to shoot or release into the rhizosphere. They are involved in root development, plant -microbes interaction in rhizosphere by promoting growth of arbuscular mycorrhiza fungi, also poviding mineral nutrients, nitrogen fixation and nodulations in legumes. Analysis of strigolactones is challenging because of its low amount (15–30 pg/plant/day) and interference created by primary metabolites, co-presence of structural analogs and highly complex plant matrix. The current analytical procedures adopted for strigolactones isolation, purification, and characterization are non-specific, time consuming with absolute requirement of high sample amount. Development of simple, rapid, reliable, and accurate protocol for strigolactones analysis will be very useful to initiate further research on different aspects of this hormone. The source for strigolactones isolation (root exudates and root tissue) are important factors for further selection of analytical methods. Solvent extraction, solid phase extraction, high performance liquid chromatography (HPLC), gas chromatography (GC), mass spectrometer (MS) are some of the important methods associated with strigolactones analysis. Some new tools such as HR-DART-MS (direct analysis in real time) and DESI-MS (desorption electrospray ionization) reported to reduce sample size significantly and yield results directly using crude extract.
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Borase, H.P., Patil, S.V., Amin, D. (2022). Strigolactones: Extraction and Characterization . In: Amaresan, N., Patel, P., Amin, D. (eds) Practical Handbook on Agricultural Microbiology. Springer Protocols Handbooks. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1724-3_38
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DOI: https://doi.org/10.1007/978-1-0716-1724-3_38
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