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Detailed Methodology to Establish a Synovium/Cartilage-on-a-Chip Model to Investigate the Process of Monocyte Extravasation

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Organ-on-a-Chip

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2373))

Abstract

Microfluidics allows for recapitulating organotypic environments in miniaturized cell culture platforms. This ability paves the way to the investigation of complex biological processes in a relevant milieu. Here we describe the protocols to generate an organotypic model including a vascularized compartment mimicking the synovial membrane and designed for the study of monocyte extravasation during osteoarthritis.

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References

  1. Rahmati M, Mobasheri A, Mozafari M (2016) Inflammatory mediators in osteoarthritis: a critical review of the state-of-the-art, current prospects, and future challenges. Bone 85:81–90. https://doi.org/10.1016/j.bone.2016.01.019

    Article  CAS  PubMed  Google Scholar 

  2. Buckwalter JA, Martin JA (2006) Osteoarthritis. Adv Drug Deliv Rev 58(2):150–167. https://doi.org/10.1016/j.addr.2006.01.006

    Article  CAS  PubMed  Google Scholar 

  3. Aspden RM, Saunders FR (2019) Osteoarthritis as an organ disease: from the cradle to the grave. European Cells Mater 37:74–87. https://doi.org/10.22203/eCM.v037a06

    Article  CAS  Google Scholar 

  4. Scanzello CR, Goldring SR (2012) The role of synovitis in osteoarthritis pathogenesis. Bone 51(2):249–257. https://doi.org/10.1016/j.bone.2012.02.012

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  5. Mathiessen A, Conaghan PG (2017) Synovitis in osteoarthritis: current understanding with therapeutic implications. Arthritis Res Ther 19(1):18. https://doi.org/10.1186/s13075-017-1229-9

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  6. Sokolove J, Lepus CM (2013) Role of inflammation in the pathogenesis of osteoarthritis: latest findings and interpretations. Ther Adv Musculoskelet Dis 5(2):77–94. https://doi.org/10.1177/1759720X12467868

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  7. Coughlin MF, Kamm RD (2020) The use of microfluidic platforms to probe the mechanism of cancer cell extravasation. Adv Healthcare Mater 9:e1901410. https://doi.org/10.1002/adhm.201901410

    Article  CAS  Google Scholar 

  8. Mondadori C, Palombella S, Salehi S et al (2021) Recapitulating monocyte extravasation to the synovium in an organotypic microfluidic model of the articular joint. Biofabrication 13(4):045001

    Google Scholar 

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Acknowledgments

This project was funded by the Italian Ministry of Health (PE-2013-02356613).

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Author notes

  1. Silvia Palombella and Shima Salehi contributed equally to this work.

Authors and Affiliations

  1. IRCCS Istituto Ortopedico Galeazzi, Cell and Tissue Engineering Laboratory, Milan, Italy

    Silvia Palombella, Shima Salehi, Carlotta Mondadori, Giuseppe Talò, Matteo Moretti & Silvia Lopa

  2. IRCCS Istituto Ortopedico Galeazzi, Milan, Italy

    Valerio Sansone

  3. Regenerative Medicine Technologies Lab, Ente Ospedaliero Cantonale, Lugano, Switzerland

    Matteo Moretti

  4. Euler Institute, Faculty of Biomedical Sciences, Università della Svizzera Italiana, Lugano, Switzerland

    Matteo Moretti

Authors
  1. Silvia Palombella
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  2. Shima Salehi
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  3. Carlotta Mondadori
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  4. Giuseppe Talò
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  5. Valerio Sansone
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  6. Matteo Moretti
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  7. Silvia Lopa
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Corresponding author

Correspondence to Silvia Lopa .

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Editors and Affiliations

  1. Department of Electronics, Information and Bioengineering, Politecnico di Milano, Milano, Italy

    Marco Rasponi

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Palombella, S. et al. (2022). Detailed Methodology to Establish a Synovium/Cartilage-on-a-Chip Model to Investigate the Process of Monocyte Extravasation. In: Rasponi, M. (eds) Organ-on-a-Chip. Methods in Molecular Biology, vol 2373. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1693-2_15

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  • DOI: https://doi.org/10.1007/978-1-0716-1693-2_15

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1692-5

  • Online ISBN: 978-1-0716-1693-2

  • eBook Packages: Springer Protocols

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