Abstract
Parkinson’s disease (PD) is a neurodegenerative disorder that affects the motor system. PD is characterized by the accumulation of intracellular protein aggregates, Lewy bodies, and Lewy neurites, composed primarily of the protein α-synuclein. Thus, PD is classified as the most common synucleinopathy. The motor symptoms of the disease result from the death of cells in the region of the midbrain, leading to a dopamine deficit. While the cause of PD is unknown, it is believed to involve both inherited and environmental factors. PD has been extensively studied using in vitro and in vivo models; however, some discrepancy is observed in these results. In order to analyze progressive neurodegenerative disease, experimental platform amenable to continuous observation and experimental manipulation is required. In this chapter, we provide a practical method to slice and cultivate the midbrain tissue as an ex vivo experimental model.
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Acknowledgments
This work was supported by JSPS KAKENHI Grant Number JP20K07765 and JP17K09040 (to Y.K), and grants-in-aid from the TERUMO LIFE SCIENCE FOUNDATION (to Y.K).
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Kamikubo, Y., Wakisaka, K., Imai, Y., Sakurai, T. (2021). Midbrain Slice Culture as an Ex Vivo Analysis Platform for Parkinson’s Disease. In: Imai, Y. (eds) Experimental Models of Parkinson’s Disease. Methods in Molecular Biology, vol 2322. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1495-2_11
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DOI: https://doi.org/10.1007/978-1-0716-1495-2_11
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