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Pathway Engineering for Beta-Carotene and Carotenoid Biosynthesis in Y. lipolytica

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Yarrowia lipolytica

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2307))

Abstract

β-carotene is an increasingly sought-after organic pigment with antioxidant properties and a vitamin precursor. Yarrowia lipolytica, though unable to naturally synthesize carotenoids, can produce high amounts of the precursor acetyl-CoA making it a promising host for metabolic engineering towards novel biotechnological production of carotenoids. Here, we describe a synthetic biology methodology for Y. Lipolytica metabolic engineering based on Golden Gate DNA assembly for the generation of a multigene cassette, subsequent transformation enabling β-carotene biosynthesis, and quantification of the compound.

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Acknowledgments

R. Ledesma-Amaro received financial support from the Imperial College London in the form of an IC Research Fellowship.

Author information

Authors and Affiliations

  1. Department of Bioengineering and Imperial College Centre for Synthetic Biology, Imperial College London, London, UK

    Mauricio Pesantes-Munoz & Rodrigo Ledesma-Amaro

Authors
  1. Mauricio Pesantes-Munoz
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  2. Rodrigo Ledesma-Amaro
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Corresponding author

Correspondence to Rodrigo Ledesma-Amaro .

Editor information

Editors and Affiliations

  1. Chemical and Environmental Engineering, University of California Riverside, Riverside, CA, USA

    Ian Wheeldon

  2. Department of Chemical and Biomolecular Engineering, Clemson University, Clemson, SC, USA

    Mark Blenner

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Pesantes-Munoz, M., Ledesma-Amaro, R. (2021). Pathway Engineering for Beta-Carotene and Carotenoid Biosynthesis in Y. lipolytica. In: Wheeldon, I., Blenner, M. (eds) Yarrowia lipolytica. Methods in Molecular Biology, vol 2307. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1414-3_13

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  • DOI: https://doi.org/10.1007/978-1-0716-1414-3_13

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1413-6

  • Online ISBN: 978-1-0716-1414-3

  • eBook Packages: Springer Protocols

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