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Engineering Yarrowia lipolytica for Production of Fatty Alcohols with YaliBrick Vectors

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Yarrowia lipolytica

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2307))

Abstract

Biosynthesis of fatty alcohol holds great promise as substitute to replace petroleum-derived fatty alcohols to mitigate environmental concerns and reduce earth’s carbon footprint. In this protocol, we detail the procedures of how to use the YaliBrick gene assembly platform to achieve modular assembly of fatty alcohol pathway in Y. lipolytica. To limit fatty alcohol oxidation, we will also describe the hydroxyurea-based protocols for the efficient disruption of POX1 gene, encoding the fatty acyl coenzyme A in Y. lipolytica, with the homologous arm about 500 bp. We envision that this chapter would improve our ability to engineer microbial cell factories for oleochemical and fatty alcohol production in oleaginous yeast species.

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Author notes

  1. Wanqi Sun and Zhiliang Yang contributed equally to this work.

Authors and Affiliations

  1. Department of Chemical, Biochemical and Environmental Engineering, University of Maryland Baltimore County, Baltimore, MD, USA

    Wanqi Sun, Zhiliang Yang & Peng Xu

Authors
  1. Wanqi Sun
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  2. Zhiliang Yang
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  3. Peng Xu
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Corresponding author

Correspondence to Peng Xu .

Editor information

Editors and Affiliations

  1. Chemical and Environmental Engineering, University of California Riverside, Riverside, CA, USA

    Ian Wheeldon

  2. Department of Chemical and Biomolecular Engineering, Clemson University, Clemson, SC, USA

    Mark Blenner

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Sun, W., Yang, Z., Xu, P. (2021). Engineering Yarrowia lipolytica for Production of Fatty Alcohols with YaliBrick Vectors. In: Wheeldon, I., Blenner, M. (eds) Yarrowia lipolytica. Methods in Molecular Biology, vol 2307. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1414-3_11

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  • DOI: https://doi.org/10.1007/978-1-0716-1414-3_11

  • Published:

  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1413-6

  • Online ISBN: 978-1-0716-1414-3

  • eBook Packages: Springer Protocols

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