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Fundamental and Practical Aspects in the Formulation of Colloidal Polyelectrolyte Complexes of Chitosan and siRNA

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Design and Delivery of SiRNA Therapeutics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2282))

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Abstract

The formation of electrostatic interactions between polyanionic siRNA and polycations gives an easy access to the formation of colloidal particles capable of delivering siRNA in vitro or in vivo. Among the polycations used for siRNA delivery, chitosan occupies a special place due to its unique physicochemical and biological properties. In this chapter we describe the fundamental and practical aspects of the formation of colloidal complexes between chitosan and siRNA. The basis of the electrostatic complexation between oppositely charged polyelectrolytes is first introduced with a focus on the specific conditions to obtain stable colloid complex particles. Subsequent, the properties that make chitosan so special are described. In a third part, the main parameters influencing the colloidal properties and stability of siRNA/chitosan complexes are reviewed with emphasis on some practical aspects to consider in the preparation of complexes.

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Authors and Affiliations

  1. Laboratoire de Chimie des Polymères Organiques (LCPO), University of Bordeaux, CNRS, Bordeaux INP, UMR 5629, Pessac, France

    Christophe Schatz & Tim Delas

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  1. Christophe Schatz
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  2. Tim Delas
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Correspondence to Tim Delas .

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  1. Department of Oncology, Odense University Hospital Odense, Denmark, Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark

    Henrik J. Ditzel

  2. Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark

    Martina Tuttolomondo

  3. Center for RNA Medicine, Department of Clinical Medicine, Aalborg University, Copenhagen, Denmark

    Sakari Kauppinen

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Schatz, C., Delas, T. (2021). Fundamental and Practical Aspects in the Formulation of Colloidal Polyelectrolyte Complexes of Chitosan and siRNA. In: Ditzel, H.J., Tuttolomondo, M., Kauppinen, S. (eds) Design and Delivery of SiRNA Therapeutics. Methods in Molecular Biology, vol 2282. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1298-9_17

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  • DOI: https://doi.org/10.1007/978-1-0716-1298-9_17

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