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siRNA Design and Delivery Based on Carbon Nanotubes

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Design and Delivery of SiRNA Therapeutics

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2282))

Abstract

Owing to the unique physical and chemical properties of carbon nanotubes, they have been widely explored as delivery vectors for proteins, and nucleic acid etc. after functionalization. Particularly, the modification of carbon nanotubes suited for the delivery of siRNA has been intensely studied over the past decade. The assay described in this chapter allows for realizable quantification of siRNA binding on carbon nanotube-based materials using gel electrophoresis and silencing by flow cytometry when the siRNA complexes are delivered in vitro.

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Author information

Authors and Affiliations

  1. School of Cancer and Pharmaceutical Sciences, King’s College London, London, UK

    Danyang Li & Khuloud T. Al-Jamal

Authors
  1. Danyang Li
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  2. Khuloud T. Al-Jamal
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Corresponding author

Correspondence to Khuloud T. Al-Jamal .

Editor information

Editors and Affiliations

  1. Department of Oncology, Odense University Hospital Odense, Denmark, Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark

    Henrik J. Ditzel

  2. Department of Cancer and Inflammation Research, Institute of Molecular Medicine, University of Southern Denmark, Odense, Denmark

    Martina Tuttolomondo

  3. Center for RNA Medicine, Department of Clinical Medicine, Aalborg University, Copenhagen, Denmark

    Sakari Kauppinen

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Li, D., Al-Jamal, K.T. (2021). siRNA Design and Delivery Based on Carbon Nanotubes. In: Ditzel, H.J., Tuttolomondo, M., Kauppinen, S. (eds) Design and Delivery of SiRNA Therapeutics. Methods in Molecular Biology, vol 2282. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1298-9_12

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  • DOI: https://doi.org/10.1007/978-1-0716-1298-9_12

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-1297-2

  • Online ISBN: 978-1-0716-1298-9

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