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Redox Titration of Flavoproteins: An Overview

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Flavins and Flavoproteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2280))

Abstract

Redox titration of flavoproteins allows to detect and analyze (1) the determinants of the stabilization of individual redox forms of the flavin by the protein; (2) the binding of the redox-active cofactor to the protein; (3) the effects of other components of the systems (such as micro- or macromolecular interactors) on parameters 1 and 2; (4) the pattern of electron flow to and from the flavin cofactor to other redox-active chemical species, including those present in the protein itself or in its physiological partners. This overview presents and discusses the fundamentals of the methodological approaches most commonly used for these purposes, and illustrates how data may be obtained in a reliable way, and how they can be read and interpreted.

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Authors and Affiliations

  1. Section of Chemical and Biomolecular Sciences, DeFENS, University of Milan, Milan, Italy

    Francesco Bonomi & Stefania Iametti

Authors
  1. Francesco Bonomi
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  2. Stefania Iametti
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Correspondence to Francesco Bonomi .

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Editors and Affiliations

  1. Department of Biosciences, Biotechnology and Biopharmaceutics, University of Bari “A. Moro”, Bari, Italy

    Maria Barile

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Bonomi, F., Iametti, S. (2021). Redox Titration of Flavoproteins: An Overview. In: Barile, M. (eds) Flavins and Flavoproteins. Methods in Molecular Biology, vol 2280. Springer, New York, NY. https://doi.org/10.1007/978-1-0716-1286-6_8

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  • DOI: https://doi.org/10.1007/978-1-0716-1286-6_8

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  • Publisher Name: Springer, New York, NY

  • Print ISBN: 978-1-0716-1285-9

  • Online ISBN: 978-1-0716-1286-6

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