Abstract
Bench-to-bedside axis of therapeutic product development is currently being oriented towards minimum invasiveness on both ends—not only clinical application but harvesting of the starting biological material as well. This is particularly relevant for Advanced Therapy Medicinal Products and their specific legislative requirements, even more so in skin regeneration. It is precisely the skin equivalents and grafts that benefit from the minimum-to-noninvasive approach to a noteworthy extent, taking in account the sensitive nature of both skin harvesting and grafting.
This chapter includes protocols for two separate steps of generating skin equivalent from the cells cultured from hair follicle outer root sheath. The first step is a non-pigmented epidermal equivalent generated from human keratinocytes from the outer root sheath named non-pigmented epidermal graft. The second step consists of co-cultivating human keratinocytes and human melanocytes from the outer root sheath, hereby producing a pigmented epidermal graft.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Dieckmann C, Renner R, Milkova L et al (2010) Regenerative medicine in dermatology: biomaterials, tissue engineering, stem cells, gene transfer and beyond. Exp Dermatol 19(8):697–706. https://doi.org/10.1111/j.1600-0625.2010.01087.x
Limat A, Hunziker T (2002) Use of epidermal equivalents generated from follicular outer root sheath cells in vitro and for autologous grafting of chronic wounds. Cells Tissues Organs 172(2):79–85. https://doi.org/10.1159/000065615
Anderer U, Savkovic V (2015) Zelltherapien in der Regenerativen Medizin. In: Sack U (ed) Zelluläre Diagnostik und Therapie. De Gruyter, Berlin, pp 291–341
Savkovic V, Dieckmann C, Milkova L et al (2012) Improved method of differentiation, selection and amplification of human melanocytes from the hair follicle cell pool. Exp Dermatol 21(12):948–950. https://doi.org/10.1111/exd.12038
Schneider M, Dieckmann C, Rabe K et al (2014) Differentiating the stem cell pool of human hair follicle outer root sheath into functional melanocytes. Methods Mol Biol 1210:203–227. https://doi.org/10.1007/978-1-4939-1435-7_16
Fitzpatrick TB, Breathnach AS (1963) The epidermal melanin unit system. Dermatol Wochenschr 147:481–489
Schneider M, Ziemer M, Simon JC et al (2020) Generation of pigmented and dermoepidermal skin grafts from human hair follicles. Tiss. Eng. A (in Revision)
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2021 Springer Science+Business Media, LLC, part of Springer Nature
About this protocol
Cite this protocol
Savkovic, V., Schneider, M., Li, H., Simon, JC., Ziemer, M., Lethaus, B. (2021). Generation of Epidermal Equivalents from Hair Follicle Melanocytes, Keratinocytes, and Dermal Fibroblasts. In: Stock, P., Christ, B. (eds) In Vitro Models for Stem Cell Therapy. Methods in Molecular Biology, vol 2269. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-1225-5_13
Download citation
DOI: https://doi.org/10.1007/978-1-0716-1225-5_13
Published:
Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-1224-8
Online ISBN: 978-1-0716-1225-5
eBook Packages: Springer Protocols