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Statistical and Bioinformatics Analysis of Data from Bulk and Single-Cell RNA Sequencing Experiments

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Translational Bioinformatics for Therapeutic Development

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2194))

Abstract

High-throughput sequencing (HTS) has revolutionized researchers’ ability to study the human transcriptome, particularly as it relates to cancer. Recently, HTS technology has advanced to the point where now one is able to sequence individual cells (i.e., “single-cell sequencing”). Prior to single-cell sequencing technology, HTS would be completed on RNA extracted from a tissue sample consisting of multiple cell types (i.e., “bulk sequencing”). In this chapter, we review the various bioinformatics and statistical methods used in the processing, quality control, and analysis of bulk and single-cell RNA sequencing methods. Additionally, we discuss how these methods are also being used to study tumor heterogeneity.

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  1. Department of Biostatistics and Bioinformatics, H. Lee Moffitt Cancer Center & Research Institute, Tampa, FL, USA

    Xiaoqing Yu, Farnoosh Abbas-Aghababazadeh, Y. Ann Chen & Brooke L. Fridley

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Yu, X., Abbas-Aghababazadeh, F., Chen, Y.A., Fridley, B.L. (2021). Statistical and Bioinformatics Analysis of Data from Bulk and Single-Cell RNA Sequencing Experiments. In: Markowitz, J. (eds) Translational Bioinformatics for Therapeutic Development. Methods in Molecular Biology, vol 2194. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0849-4_9

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