Abstract
Virus-induced gene silencing (VIGS) is a favorable method to study gene function by posttranscriptional gene silencing in plants. Here we describe a methodology of graft-accelerated VIGS in rose aimed at obtaining posttranscriptional gene silencing in the flower. The resulting phenotype can be observed within 5–6 weeks post infiltration. By using this method, we successfully silenced the expression of several genes involved in processes such as scent production, petal coloration, or flower architecture. We showed that graft-accelerated VIGS was faster, more efficient, and more convenient than conventional methods previously developed in rose such as agroinfiltration of young plantlets and in vitro cultured tissues or seeds.
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Acknowledgments
This work was supported by the National Natural Science Foundation of China (Grants numbers 31660579, 31872144, and 31501791). This research was funded in part by the Beijing Natural Science Foundation (6162017).
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Yan, H., Zhang, Z., Magnard, JL., Boachon, B., Baudino, S., Tang, K. (2020). Virus-Induced Gene Silencing in Rose Flowers. In: Courdavault, V., Besseau, S. (eds) Virus-Induced Gene Silencing in Plants. Methods in Molecular Biology, vol 2172. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0751-0_16
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DOI: https://doi.org/10.1007/978-1-0716-0751-0_16
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