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Interrogating Membrane Protein Structure and Lipid Interactions by Native Mass Spectrometry

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Biophysics of Membrane Proteins

Part of the book series: Methods in Molecular Biology ((MIMB,volume 2168))

Abstract

Native mass spectrometry and native ion mobility mass spectrometry are now established techniques in structural biology, with recent work developing these methods for the study of integral membrane proteins reconstituted in both lipid bilayer and detergent environments. Here we show how native mass spectrometry can be used to interrogate integral membrane proteins, providing insights into conformation, oligomerization, subunit composition/stoichiometry, and interactions with detergents/lipids/drugs. Furthermore, we discuss the sample requirements and experimental considerations unique to integral membrane protein native mass spectrometry research.

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Acknowledgments

D.H., J.F.V.D., and F.S. acknowledge the Antwerp University Research Fund for the Concerted Research Actions grant (BOF-GOA 4D protein structure). A.N.C. acknowledges funding from the BBSRC (BB/P000037/1) and support from a University Academic Fellowship from the University of Leeds.

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Authors and Affiliations

  1. Protein Chemistry, Proteomics and Epigenetic Signalling (PPES), Department of Biomedical Sciences, University of Antwerp, Wilrijk, Belgium

    Dietmar Hammerschmid

  2. Biomolecular & Analytical Mass Spectrometry Group, Chemistry Department, University of Antwerp, Antwerp, Belgium

    Dietmar Hammerschmid, Jeroen F. van Dyck & Frank Sobott

  3. Faculty of Biological Sciences, School of Molecular and Cellular Biology, University of Leeds, Leeds, UK

    Frank Sobott & Antonio N. Calabrese

  4. Astbury Centre for Structural Molecular Biology, University of Leeds, Leeds, UK

    Frank Sobott & Antonio N. Calabrese

Authors
  1. Dietmar Hammerschmid
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  2. Jeroen F. van Dyck
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  3. Frank Sobott
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  4. Antonio N. Calabrese
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Corresponding author

Correspondence to Antonio N. Calabrese .

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Editors and Affiliations

  1. School of Clinical and Applied Sciences, Leeds Beckett University, Leeds, UK

    Vincent L. G. Postis

  2. Astbury Centre for Structural Molecular Biology, School of Biomedical Sciences; Molecular and Integrative Biosciences, Faculty of Biological and Environmental Sciences, University of Leeds, Leeds, UK; University of Helsinki, Helsinki, Finland

    Adrian Goldman

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Hammerschmid, D., van Dyck, J.F., Sobott, F., Calabrese, A.N. (2020). Interrogating Membrane Protein Structure and Lipid Interactions by Native Mass Spectrometry. In: Postis, V.L.G., Goldman, A. (eds) Biophysics of Membrane Proteins. Methods in Molecular Biology, vol 2168. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-0724-4_11

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  • DOI: https://doi.org/10.1007/978-1-0716-0724-4_11

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  • Publisher Name: Humana, New York, NY

  • Print ISBN: 978-1-0716-0723-7

  • Online ISBN: 978-1-0716-0724-4

  • eBook Packages: Springer Protocols

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