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Derivation of Neural Stem Cells from Mouse Induced Pluripotent Stem Cells

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Induced Pluripotent Stem (iPS) Cells

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1357))

Abstract

Neural stem cells (NSCs) derived from induced pluripotent stem cells offer therapeutic tools for neurodegenerative diseases. This review focuses on embryoid body (EB)-mediated stem cell culture techniques used to derive NSCs from mouse induced pluripotent stem cells (iPSCs). Generation of healthy and stable NSCs from iPSCs heavily depends on standardized in vitro cell culture systems that mimic the embryonic environments utilized during neural development. Specifically, the neural induction and expansion methods after EB formation are described in this review.

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Acknowledgements

This work is supported by FP7 Marie Curie CIG and Koç University School of Medicine.

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Authors and Affiliations

  1. Brain Cancer Research and Therapy Laboratory, Koç University School of Medicine, Rumelifeneri Yolu, Sarıyer, Istanbul, 34450, Turkey

    Işıl Karanfil & Tugba Bagci-Onder

Authors
  1. Işıl Karanfil
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  2. Tugba Bagci-Onder
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Corresponding author

Correspondence to Tugba Bagci-Onder .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

  2. Mount Sinai Hospital Samuel Lunenfeld Research Inst, Toronto, Ontario, Canada

    Andras Nagy

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Karanfil, I., Bagci-Onder, T. (2015). Derivation of Neural Stem Cells from Mouse Induced Pluripotent Stem Cells. In: Turksen, K., Nagy, A. (eds) Induced Pluripotent Stem (iPS) Cells. Methods in Molecular Biology, vol 1357. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_227

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  • DOI: https://doi.org/10.1007/7651_2015_227

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-3054-8

  • Online ISBN: 978-1-4939-3055-5

  • eBook Packages: Springer Protocols

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