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Generation and Purification of Definitive Endoderm Cells Generated from Pluripotent Stem Cells

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Embryonic Stem Cell Protocols

Part of the book series: Methods in Molecular Biology ((MIMB,volume 1341))

Abstract

Differentiation of pluripotent stem cells into cells of the definitive endoderm requires an in vitro gastrulation event. Differentiated somatic cells derived from this germ layer may then be used for cell replacement therapies of degenerative diseases of the liver, lung, and pancreas. Here we describe an endoderm differentiation protocol, which initiates the differentiation from a defined cell number of dispersed single cells and reliably yields in >70–80 % endoderm-committed cells in a short 5-day treatment regimen.

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Authors and Affiliations

  1. Institute of Clinical Biochemistry, Hannover Medical School, 30625, Hannover, Germany

    Ulf Diekmann & Ortwin Naujok

Authors
  1. Ulf Diekmann
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  2. Ortwin Naujok
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Corresponding author

Correspondence to Ortwin Naujok .

Editor information

Editors and Affiliations

  1. Ottawa Hospital Research Institute, Ottawa, Ontario, Canada

    Kursad Turksen

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© 2015 Springer Science+Business Media New York

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Diekmann, U., Naujok, O. (2015). Generation and Purification of Definitive Endoderm Cells Generated from Pluripotent Stem Cells. In: Turksen, K. (eds) Embryonic Stem Cell Protocols. Methods in Molecular Biology, vol 1341. Humana Press, New York, NY. https://doi.org/10.1007/7651_2015_220

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  • DOI: https://doi.org/10.1007/7651_2015_220

  • Published:

  • Publisher Name: Humana Press, New York, NY

  • Print ISBN: 978-1-4939-2953-5

  • Online ISBN: 978-1-4939-2954-2

  • eBook Packages: Springer Protocols

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