Abstract
Direct reprogramming of somatic cells into induced pluripotent stem cells (iPSCs) provides an opportunity to develop novel personalized treatment options for numerous diseases and to advance current approaches for cell-based drug discoveries and disease modeling. The ability to differentiate iPSCs into relevant cell types is an important prerequisite for the successful development of iPSC-based treatment and modeling strategies. Here, we describe a protocol for the efficient differentiation of human iPSCs into functional keratinocytes. The protocol employs treating iPSCs with retinoic acid and bone-morphogenetic protein-4 to induce differentiation toward a keratinocyte lineage, which is then followed by the growth of differentiated iPSCs on collagen type I- and collagen type IV-coated dishes to enrich for iPSC-derived keratinocytes.
An erratum to this chapter can be found at http://dx.doi.org/10.1007/7651_2014_79
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Abbreviations
- ColI:
-
Type I collagen
- ColIV:
-
Type IV collagen
- ESC:
-
Embryonic stem cell
- iPSC:
-
Induced pluripotent stem cell
- Krt14:
-
Keratin 14
- RA:
-
Retinoic acid
- BMP4:
-
Bone-morphogenetic protein-4
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Acknowledgements
We are grateful for funding support from the National Institutes of Health (R01AR059947 and P30 AR057212), the US Department of Defense (PR110793), the Foundation for Ichthyosis and Related Skin Types (F.I.R.S.T.), and the Dystrophic Epidermolysis Bullosa Research Association (DEBRA) International.
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Kogut, I., Roop, D.R., Bilousova, G. (2013). Differentiation of Human Induced Pluripotent Stem Cells into a Keratinocyte Lineage. In: Turksen, K. (eds) Epidermal Cells. Methods in Molecular Biology, vol 1195. Springer, New York, NY. https://doi.org/10.1007/7651_2013_64
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DOI: https://doi.org/10.1007/7651_2013_64
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