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Advanced FCS: An Introduction to Fluorescence Lifetime Correlation Spectroscopy and Dual-Focus FCS

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Advanced Photon Counting

Part of the book series: Springer Series on Fluorescence ((SS FLUOR,volume 15))

Abstract

This chapter focuses on two advanced fluorescence correlation spectroscopy (FCS) methods; fluorescence lifetime correlation spectroscopy (FLCS) and dual-focus FCS (2fFCS). We decided to put our focus on a detailed discussion of these two – and in our eyes well-merited – advanced methods, rather than giving an overview over the broad variety of advanced FCS methods that would consequently lack detail and leave the reader rather uneducated on all these methods. For this reason we had to exclude some candidates that would very well deserve the same amount of attention as the methods that we chose to focus on. Amongst these candidates camera-FCS, Bayes-FCS, and scanning-FCS are to be kept on the radar for sure.

The great benefit of FLCS is that it provides a general tool that allows filtering for sub-populations, afterpulsing-artifacts, background effects, and basically anything that can be distinguished by its fluorescence lifetime. Complementarily, 2fFCS has brought a new level of accuracy to the table that has been previously reached only by complementary methods such as for example pulsed-field gradient NMR.

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Authors and Affiliations

  1. SOFast GmbH, Berlin, Germany

    Thomas Dertinger

  2. PicoQuant Innovations GmbH, Berlin, Germany

    Steffen Rüttinger

Authors
  1. Thomas Dertinger
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  2. Steffen Rüttinger
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Correspondence to Thomas Dertinger .

Editor information

Editors and Affiliations

  1. J. Heyrovsky Institute of Physical Chemistry, Academy of Sciences of the Czech Republic, Prague 8, Czech Republic

    Peter Kapusta

  2. PicoQuant GmbH, Berlin, Germany

    Michael Wahl

  3. PicoQuant GmbH, Berlin, Germany

    Rainer Erdmann

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© 2014 Springer International Publishing Switzerland

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Dertinger, T., Rüttinger, S. (2014). Advanced FCS: An Introduction to Fluorescence Lifetime Correlation Spectroscopy and Dual-Focus FCS. In: Kapusta, P., Wahl, M., Erdmann, R. (eds) Advanced Photon Counting. Springer Series on Fluorescence, vol 15. Springer, Cham. https://doi.org/10.1007/4243_2014_72

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