Skip to main content
SpringerLink
Log in

Are the profilins an important component in the atmosphere? Ole e 2-like panallergen

  • Original Paper
  • Published:
Aerobiologia Aims and scope Submit manuscript

Abstract

The analysis of profilin Ole e 2 concentration and its relationship with pollen concentration was carried out in the atmosphere of León (located in north-western of Spain) during 3 years. Moreover, we have studied the influence of some atmospheric pollutants and climatic parameters using Spearman rank correlation and principal components analysis. As a result, the presence in the atmosphere of Ole e 2 is demonstrated. We can observe that in April and until mid-May, the peaks of Ole e 2 correspond to the high concentrations of pollen from various tree plants, such as Quercus, Populus, Betula, Cupressaceae. From this date until the middle of June, where the presence of this profilin begins to be scarce in the air, the concentration of Ole e 2 is linked to herbaceous plants: Rumex, Plantago and species of the family Poaceae. Statistical analysis showed that the factors of temperature get influenced the most in the presence of Ole e 2 in the atmosphere, followed by the relative humidity and precipitation. In our study, there was no clear correlation with atmospheric pollutants; however, an increase in NO2 and SO2 causes a decrease in profilin in the atmosphere.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3

Similar content being viewed by others

References

  • Ackaert, C., Kofler, S., Horejs-Hoeck, J., Zulehner, N., Asam, C., von Grafenstein, S., et al. (2014). The impact of nitration on the structure and immunogenicity of the major birch pollen allergen Bet v 1.0101. PLoS ONE, 9(8), e104520.

    Article  CAS  Google Scholar 

  • Alché, J. D., M’rani-Alaoui, M., Castro, A. J., & Rodríguez-García, M. I. (2004). Ole e 1, the major allergen from olive (Olea europaea L.) pollen, is newly synthesized and released to the culture medium during in vitro germination. Plant and Cell Physiology, 45(8), 1149–1157.

    Article  Google Scholar 

  • Alché, J. J., Castro, A. J., Jiménez-López, J. C., Morales, S., Zafra, A., Hamman-Khalifa, A. M., et al. (2007). Differential characteristics of Olive pollen from different cultivars: Biological and clinical implications. Journal of Investigational Allergology and Clinical Immunology, 17(1), 69–75.

    Google Scholar 

  • Aloisi, I., Del Duca, S., De Nuntiis, P., Vega Maray, A. M., Mandrioli, P., Gutiérrez, P., et al. (2018). Behavior of profilins in the atmosphere and in vitro, and their relationship with the performance of airborne pollen. Atmospheric Environment, 178, 231–241. https://doi.org/10.1016/j.atmosenv.2018.01.051.

    Article  CAS  Google Scholar 

  • Arilla, M. C., Asturias, J. A., Gómez-Bayón, N., Martínez, A., Martínez, J., & Palacios, R. (1997). Production and characterization of profilin monoclonal antibodies. Allergologie et Immunopathologie, 25, 145–151.

    CAS  Google Scholar 

  • Asturias, J. A., Arilla, M. C., Aguirre, M., Gómez-Bayón, N., Martínez, A., Palacios, R., et al. (1999). Quantitification of profilins by a monoclonal antibody-based sandwich ELISA. Journal of Immunological Methods, 229, 61–71.

    Article  CAS  Google Scholar 

  • Barber, D., de la Torre, F., Feo, F., Florido, F., Guardia, P., Moreno, C., et al. (2008). Understanding patient sensitization profiles in complex pollen areas: A molecular epidemiological study. Allergy, 63(11), 1550–1558.

    Article  CAS  Google Scholar 

  • Beggs, P. J. (1998). Pollen and pollen antigen as triggers of asthma—What to mesure? Atmospheric Environment, 32(10), 1777–1783.

    Article  CAS  Google Scholar 

  • Buters, J., Prank, M., Sofiev, M., Pusch, G., Albertini, R., Annesi-Maesano, I., et al. (2015). Variation of the group 5 grass pollen allergen content of airborne pollen in relation to geographic location and time in season. Journal of Allergy and Clinical Immunology, 136(87–95), e86.

    Google Scholar 

  • Buters, J. T. M., Thibaudon, M., Smith, M., Kennedy, R., Rantio-Lehtimäki, A., Albertini, R., et al. (2012). Release of Bet v 1 from birch pollen from 5 European countries. Results from the HIALINE Study. Atmospheric Environment, 55, 496–505.

    Article  CAS  Google Scholar 

  • Chichiricco, G., & Picozzi, P. (2007). Reversible inhibition of the pollen germination and the stigma penetration in Crocus vernus ssp vernus (Iridaceae) following fumigations with NO2, CO, and O3 gases. Plant Biology, 9, 730–735.

    Article  CAS  Google Scholar 

  • Cuinica, L. C., Abreu, I., Joaquim, C. G., & da Silva, E. (2014). In vitro exposure of Ostrya carpinifolia and Carpinus betulus pollen to atmospheric levels of CO, O3 and SO2. Environmental Science and Pollution Research, 21, 2256–2262.

    Article  CAS  Google Scholar 

  • D’Amato, G., Cecchi, L., Bonini, S., Nunes, C., Annesi-Maesano, I., Behrendt, H., et al. (2007). Allergenic pollen and pollen allergy in Europe. Allergy, 62(9), 976–990.

    Article  CAS  Google Scholar 

  • D’Amato, G., de Palma, R., & Verga, A. (1991). Antigen activity of non-pollen parts (leaves and stems) of allergenic plants. Annals of Allergy, 67, 421–424.

    Google Scholar 

  • De Linares, C., Díaz de la Guardia, C., Nieto Lugilde, D., & Akba, F. (2010). Airborne study of grass allergen (Lol p 1) in different-sized particles. International Archives of Allergy and Immunology, 152, 49–57.

    Article  CAS  Google Scholar 

  • Emberlin, J. (1995). Analysis of allergens on airborne particles: Progress and problems. In Europäisches Pollenflug-Symposium (pp. 48–62). Duseldorf: Vorträge und Berichte.

  • Fernández-González, D., González-Parrado, Z., Vega-Maray, A. M., Valencia-Barrera, R. M., Camazón-Izquierdo, B., De Nuntiis, P., et al. (2010). Platanus pollen allergen, Pla a 1: Quantification in the atmosphere and influence on a sensitizing population. Clinical and Experimental Allergy, 40(11), 1701–1708.

    Article  Google Scholar 

  • Fernández-González, D., Rodríguez-Rajo, F. J., González-Parrado, Z., Valencia-Barrera, R. M., Jato, V., & Moreno-Grau, S. (2011). Differences in atmospheric emissions of Poaceae pollen and Lol p 1 allergen. Aerobiologia, 27, 301–309.

    Article  Google Scholar 

  • Ferreira, F., Hawranek, T., Gruber, P., Wopfner, N., & Mari, A. (2004). Allergic croos-reactivity: From gene to the clinic. Allergy, 59, 243–267.

    Article  CAS  Google Scholar 

  • Galán, C., Antunes, C., Brandao, R., Torres, C., García-Mozo, H., Caeiro, E., et al. (2013). Airborne olive pollen counts are not representative of exposure to the major olive allergen Ole e 1. Allergy, 68, 809–812.

    Article  Google Scholar 

  • Galán, C., Ariatti, A., Bonini, M., Clot, B., Crouzy, B., Dahl, A., et al. (2017). Recommended terminology for aerobiological studies. Aerobiologia, 33(3), 293–295.

    Article  Google Scholar 

  • Galán, C., Cariñanos, P., Alcázar, P., & Domínguez, E. (2007). Spanish aerobiology network (REA): Management and quality manual. Córdoba: Universidad de Córdoba.

    Google Scholar 

  • González-Parrado, Z., Fernándz-González, D., Camazón, B., Valencia-Barrera, R. M., Vega-Maray, A. M., Asturias, J. A., et al. (2014). Molecular aerobiology—Plantago allergen Pla l 1 in the atmosphere. Annales of Agricultural and Environmental Medicine, 21(2), 282–289.

    Article  CAS  Google Scholar 

  • Grote, M., Vrtala, S., & Valenta, R. (1993). Monitoring of two allergens, Bet v I and profilin, in dry and rehydrated birch pollen by immunogold electron microscopy and immunoblotting. Journal of Histochemistry and Cytochemistry, 41(5), 745–750.

    Article  CAS  Google Scholar 

  • Jimenez-Lopez, J. C., Morales, S., Castro, A. J., Volkmann, D., Rodríguez-García, M. I., & Alché, J. D. (2012). Characterization of profilin polymorphism in pollen with a focus on multifunctionality. PLoS ONE, 7(2), e30878.

    Article  CAS  Google Scholar 

  • Jímenez-López, J. C., Rodríguez-García, M. I., & Alché, J. D. (2013). Analysis of the effects of polymorphism on pollen profiling structural functionality and the generation of conformational, T- and B-cell epitopes. PLoS ONE, 8(10), E76066.

    Article  CAS  Google Scholar 

  • Kandasamy, M. K., McKinney, E. C., & Meagher, R. B. (2002). Plant profilin isovariants are distinctly regulated in vegetative and reproductive tissues. Cell Motility and the Cytoskeleton, 52, 22–32.

    Article  CAS  Google Scholar 

  • Laskin, A., Gilles, M. K., Knopf, D. A., Wang, B., & China, S. (2016). Progress in the analysis of complex atmospheric particles. Annual Review of Analytical Chemistry, 9(1), 117–143.

    Article  Google Scholar 

  • Ledesma, A., Rodriguez, R., & Villalba, M. (1998). Olive-pollen profilin. Molecular and immunologic properties. Allergy, 53(5), 520–526.

    Article  CAS  Google Scholar 

  • Lippmann, M., & Chan, T. L. (1979). Cyclone sampler performance. In Symposium on advances in particle sampling and measurement (Vol. 39, pp. 7–11). Asheville: Staub - Reinhaltung der Luft.

  • Morales, S., Jiménez-López, J. C., Castro, A. J., Rodríguez-García, M. I., & Alché, J. D. (2008). Olive pollen profilin (Ole e 2 allergen) co-localizes with highly active areas of the actin cytoskeleton and is released to the culture medium during in vitro pollen germination. Journal of Microscopy, 23(2), 332–341.

    Article  Google Scholar 

  • Moreno-Grau, S., Elvira-Rendueles, B., Moreno, J., Garcia-Sanchez, A., Vergara, N., Asturias, J. A., et al. (2006). Correlation between Olea europaea and Parietaria judaica pollen counts and quantification of their major allergens Ole e 1 and Par j 1-Par j 2. Annals of Allergy, Asthma & Immunology, 96, 858–864.

    Article  Google Scholar 

  • Plaza, M. P., Alcázar, P., Hernández-Ceballos, M. A., & Galán, C. (2016). Mismatch in aeroallergens and airborne grass pollen concentrations. Atmospheric Environment, 144, 361–369.

    Article  CAS  Google Scholar 

  • Rantio-Lehtimakï, A., Viander, M., & Koivikko, A. (1994). Airborne birch pollen antigens in different particle sizes. Clinical and Experimental Allergy, 24(1), 23–28.

    Article  Google Scholar 

  • Raulf, M., Buters, J. T. M., Chapman, M., Cecchi, L., de Blay, F., Doekes, G., et al. (2014). Monitoring of occupational and environmental aeroallergens—EAACI Position Paper. Concerted action of the EAACI IG Occupational allergy and serobiology & air pollution. Allergy, 69(10), 1280–1299. https://doi.org/10.1111/all.12456.

    Article  CAS  Google Scholar 

  • Reinmuth-Selzle, K., Kampf, C. J., Lucas, K., Lang-Yona, N., Frohlich-Nowoisky, J., Shiraiwa, M., et al. (2017). Air pollution and climate change effects on allergies in the anthropocene: Abundance, interaction, and modification of allergens and adjuvants. Environmental Science and Technology, 51, 4119–4141.

    Article  CAS  Google Scholar 

  • Rodríguez, R., Villalba, M., Batanero, E., González, E. M., Monsalve, R. I., Huecas, S., et al. (2002). Allergenic diversity of the olive pollen. Allergy, 57(Suppl. 71), 6–16.

    Article  Google Scholar 

  • Rothkegel, M., Mayboroda, O., Rohde, M., Wucherpfennig, C., Valenta, R., & Jockusch, B. M. (1996). Plant and animal profilins are functionally equivalent and stabilize microfilaments in living animal cells. Journal of Cell Science, 109, 83–90.

    CAS  Google Scholar 

  • Santos, A., & Van Ree, R. (2011). Profilins: Mimickers of allergy or relevant allergens? International Archives of Allergy and Immunology, 155(3), 191–204.

    Article  CAS  Google Scholar 

  • Sharma, V. K., & Graham, N. J. D. (2010). Oxidation of amino acids, peptides and proteins by ozone: A review. Ozone: Science & Engineereing, 32(2), 81–90.

    Article  CAS  Google Scholar 

  • Solomon, W. R., Burge, H. A., & Muilenberg, M. L. (1983). Allergen carriage by atmospheric aerosol, I: Ragweed pollen determinants in smaller micronic fractions. Journal of Allergy and Clinical & Immunology, 72, 443e447.

    Article  Google Scholar 

  • Sousa, R., Duque, L., Duarte, A. J., Gomes, C. R., Ribeiro, H., Cruz, A., et al. (2012). In vitro exposure of Acer negundo pollen to atmospheric levels of SO2 and NO2: Effects on allergenicity and germination. Environmental Science and Technology, 46, 2406–2412.

    Article  CAS  Google Scholar 

  • Staiger, C. J. (2000). Signaling to the actin cytoskeleton in plants. Annual Review of Plant Physiology and Plant Molecular Biology, 51, 257–288.

    Article  CAS  Google Scholar 

  • Staiger, C. J., & Blanchoin, L. (2006). Actin dynamics: Old friends with new stories. Current Opinion in Plant Biology, 9, 554–562.

    Article  CAS  Google Scholar 

  • Suárez-Cervera, M., Asturias, J., Vega Maray, A. M., Castells, T., López-Iglesias, C., Ibarrola, I., et al. (2005). The role of allergenic proteins Pla a 1 and Pla a 2 in the germination of Platanus acerifolia pollen grains. Sex Plant Reproduction, 18, 101–112.

    Article  CAS  Google Scholar 

  • Valenta, R., Duchene, M., Ebner, C., Valent, P., Sillaber, C., Deviller, F., et al. (1992). Profilins constitute a novel family of functional plant pan-allergens. Journal of Experimental Medicine, 175, 377–385.

    Article  CAS  Google Scholar 

  • Valenta, R., Duchene, M., Pettenburger, K., Sillaber, C., Valent, P., Bettelheim, P., et al. (1991). Identification of profilin as a novel pollen allergen: IgE autoreactivity in sensitized individuals. Science, 253(5019), 557–560.

    Article  CAS  Google Scholar 

  • Vara, A., Fernández-González, M., Aira, M. J., & Rodríguez-Rajo, F. J. (2016). Oleaceae cross-reactions as potential pollinosis cause in urban areas. Science of the Total Environment, 542, 435–440.

    Article  CAS  Google Scholar 

  • Vega Maray, A. M., Fernández González, D., Valencia Barrera, R. M., Polo, F., Seoane Camba, J. A., & Suárez Cervera, M. (2004). Lipid transfer proteins in Parietaria Judaica pollen grains: Immunocytochemical localization and function. European Journal of Cell Biology, 83, 493–497.

    Article  CAS  Google Scholar 

  • Velicer, W. F., & Jackson, D. N. (1990). Component analysis versus common factor analysis: Some issues in selecting an appropriate procedure. Multivariate Behavioral Research, 25, 1–28.

    Article  CAS  Google Scholar 

  • Vidali, L., & Hepler, P. K. (2001). Actin and pollen tube growth. Protoplasma, 215(1–4), 64–76.

    Article  CAS  Google Scholar 

  • Visez, N., Chassard, G., Azarkan, N., Naas, O., Sénéchal, H., Sutra, J. P., et al. (2015). Wind-induced mechanical rupture of birch pollen: Potential implications for allergen dispersal. Journal of Aerosol Science, 89, 77–84.

    Article  CAS  Google Scholar 

  • Vrtala, S., Grote, M., Duchene, M., van Ree, R., Kraft, D., Scheiner, O., et al. (1993). Properties of tree and grass pollen allergens: Reinvestigation of the linkage between solubility and allergenicity. International Archives of Allergy and Immunology, 102(2), 160–169.

    Article  CAS  Google Scholar 

  • Worden, K., & Farrar, C. R. (2007). Theme issue: Structural health monitoring. Philosophical Transactions of the Royal Society, Series A, 365(1861), 299–632.

    Google Scholar 

Download references

Acknowledgements

The authors want to thank different entities for financing this study by projects: Spanish Ministry of Science and Technology I+D+I for CGL2006-15103-C04-03 and the Castilla y León Government for SAN673-LE03-08, also the Health Department of the Castilla y León, through the RACYL-ULE.

Author information

Authors and Affiliations

  1. Department of Biodiversity and Environmental Management (Botany), University of León, León, Spain

    Delia Fernández-González, Ana Mª Vega Maray, Zulima González Parrado & Rosa Mª Valencia Barrera

  2. Department of Management and Business Economics, University of León, León, Spain

    Pablo Gutiérrez

  3. Institute of Atmospheric Sciences and Climate, National Research Council, Bologna, Italy

    Delia Fernández-González, Paola De Nuntiis & Paolo Mandrioli

Authors
  1. Delia Fernández-González
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Ana Mª Vega Maray
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Zulima González Parrado
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Rosa Mª Valencia Barrera
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Pablo Gutiérrez
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Paola De Nuntiis
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Paolo Mandrioli
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Delia Fernández-González.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Fernández-González, D., Vega Maray, A.M., González Parrado, Z. et al. Are the profilins an important component in the atmosphere? Ole e 2-like panallergen. Aerobiologia 35, 165–175 (2019). https://doi.org/10.1007/s10453-018-9548-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10453-018-9548-0

Keywords

Search

Navigation