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Glutathione S-transferase related detoxification processes are correlated with receptor-mediated vacuolar sorting mechanisms

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Abstract

Key message

Triticum durum Glutathione S-transferase Z1 is specifically responsive to glyphosate. Its expression influences the receptor-mediated vacuolar sorting mechanisms involved in tolerance mechanisms.

Abstract

A zeta subfamily glutathione S-transferase gene from Triticum durum (cv Cappelli) (TdGSTZ1) was characterized as part of a complex detoxification mechanism. The effect of different abiotic stresses on TdGSTZ1 revealed that the gene is unexpectedly responsive to glyphosate (GLY) herbicide despite it should not be part of tolerance mechanisms. Its role in the non-target-site mechanism of GLY resistance was then investigated. To analyze the GLY and the TdGSTZ1 overexpression effects on vacuolar sorting mechanisms, we performed transient transformation experiments in Nicotiana tabacum protoplasts using two vacuolar markers, AleuGFPgl133 and GFPgl133Chi, labeling the Sar1 dependent or independent sorting, respectively. We observed that the adaptive reaction of tobacco protoplasts vacuolar system to the treatment with GLY could be partially mimicked by the overexpression of TdGSTZ1 gene. To confirm the influence of GLY on the two vacuolar markers accumulation and the potential involvement of the secretion pathway activity in detoxification events, Arabidopsis thaliana transgenic plants overexpressing the non-glycosylated versions of the two markers were analyzed. The results suggested that GLY treatment specifically altered different vacuolar sorting characteristics, suggesting an involvement of the receptor-mediated AleuGFP sorting mechanism in GLY resistance. Finally, the expression analysis of selected genes confirmed that the non-target-site GLY resistance mechanisms are related to vacuolar sorting.

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Abbreviations

EPSPS:

5-Enolpyruvylshikimate-3-phosphate synthase

GLY:

Glyphosate

GSTZ:

Glutathione S-transferase

MHR:

Multiple herbicides resistance

EXPA2:

Alpha-expansin

CV:

Central vacuole

PVC:

Pre-vacuolar compartments

VSD:

C-terminal vacuolar sorting determinant

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Acknowledgements

Special thanks to the “Regione Puglia” for supporting the Project no. 14 “Reti di Laboratori Pubblici di Ricerca” “SELGE” through which a Zeiss LSM710 confocal microscope was bought.

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Authors and Affiliations

  1. DISTEBA, Department of Biological and Environmental Sciences and Technologies, University of Salento, via Prov. le Lecce-Monteroni, 73100, Lecce, Italy

    Fabrizio Barozzi, Gian-Pietro Di Sansebastiano, Erika Sabella, Alessio Aprile, Gabriella Piro, Luigi De Bellis & Eliana Nutricati

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  1. Fabrizio Barozzi
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  2. Gian-Pietro Di Sansebastiano
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  3. Erika Sabella
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  4. Alessio Aprile
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  6. Luigi De Bellis
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  7. Eliana Nutricati
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Correspondence to Eliana Nutricati.

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Communicated by Dr. Chun-Hai Dong.

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299_2017_2159_MOESM1_ESM.tiff

Figure S1. Confocal images (1.9 μm) of tobacco protoplasts transiently expressing AleuGFPgl133 in different conditions: A) treated with 0.2 mM GLY; B) co-transformed with TdGSTZ1; C) both treated with GLY and co-transformed with TdGSTZ1. In all cases, protoplasts showed more or less altered PVCs. Scale bar 20 μm (TIFF 972 kb)

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Barozzi, F., Di Sansebastiano, GP., Sabella, E. et al. Glutathione S-transferase related detoxification processes are correlated with receptor-mediated vacuolar sorting mechanisms. Plant Cell Rep 36, 1361–1373 (2017). https://doi.org/10.1007/s00299-017-2159-3

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  • DOI: https://doi.org/10.1007/s00299-017-2159-3

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