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Efficient plant regeneration from cotyledon explants of bottle gourd (Lagenaria siceraria Standl.)

  • Cell Biology and Morphogenesis
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Abstract

Using cotyledon explants excised from seedlings germinated in vitro, an efficient plant regeneration system via organogenesis was established for bottle gourd (Lagenaria siceraria Standl.). Maximum shoot regeneration was obtained when the proximal parts of cotyledons from 4-day-old seedlings were cultured on MS medium with 3 mg/l BA and 0.5 mg/l AgNO3 under a 16-h photoperiod. After 3–4 weeks of culture, 21.9–80.7% of explants from the five cultivars regenerated shoots. Adventitious shoots were successfully rooted on a half-strength MS medium with 0.1 mg/l IAA for 2–3 weeks. Flow cytometric analysis revealed that most of the regenerated plants derived from culture on medium with AgNO3 were diploid.

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Abbreviations

AVG:

l-α-(2-Aminoethoxyvinyl) glycine

BA:

6-Benzylaminopurine

IAA:

Indole-3-acetic acid

MS:

Murashige and Skoog’s

NAA:

1-Naphthaleneactic acid

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Authors and Affiliations

  1. National Horticultural Research Institute, Rural Development Administration, Suwon, 441-440, South Korea

    J-S. Han & I-G. Mok

  2. Research Management Bureau, Rural Development Administration, Suwon, 441-707, South Korea

    D-G. Oh

  3. College of Agriculture & Life Sciences, Seoul National University, Seoul, 151-742, South Korea

    H-G. Park

  4. Department of Horticulture, Sangju National University, Sangju, 742-711, South Korea

    C. K. Kim

Authors
  1. J-S. Han
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  2. D-G. Oh
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  3. I-G. Mok
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  4. H-G. Park
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  5. C. K. Kim
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Correspondence to C. K. Kim.

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Han, JS., Oh, DG., Mok, IG. et al. Efficient plant regeneration from cotyledon explants of bottle gourd (Lagenaria siceraria Standl.). Plant Cell Rep 23, 291–296 (2004). https://doi.org/10.1007/s00299-004-0846-3

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  • DOI: https://doi.org/10.1007/s00299-004-0846-3

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