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Regeneration and Analysis of Callus from Flow-Sorted Heterokaryons of Soybean (Glycine Max) and G. Canescens

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Progress in Plant Protoplast Research

Part of the book series: Current Plant Science and Biotechnology in Agriculture ((PSBA,volume 7))

Abstract

Perennial, wild Glycine spp. harbour a wealth of genetic traits useful for the agronomic improvement of the soybean (G. max). Postzygotic incompatibility has necessitated the development of techniques for hybrid embryo rescue and somatic hybridisation, via protoplast fusion, to facilitate gene flow between these species.

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References

  1. Hammatt N, Davey MR: Isolation and culture of soybean hypocotyl protoplasts. In vitro (submitted).

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  2. Hammatt N, Kim H-I, Davey MR, Nelson RS, Cocking EC: Plant regeneration from cotyledon protoplasts of Glycine canescens and G. clandestina. Plant Sci. 48, 129–135, 1987.

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  3. Watts JW, King JM: A simple method for large-scale electrofusion and culture of plant protoplasts. Biosci. Rep. 4, 335–342, 1984.

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  4. Hammatt N: Plant regeneration and protoplast fusion in the Genus Glycine. Ph.D. Thesis, Univ. Nottingham, 1986.

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  5. Schiavo FL, Guiliano G, Terzi M: Identifying natural and parasexual hybrids. In: Isozymes in Plant Genetics and Breeding, (eds.) SD Tanksley, TJ Orton, Elsevier, Amsterdam: 305–312, 1983.

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Authors and Affiliations

  1. Shell Research Ltd., Sittingbourne Research Centre, Sittingbourne, Kent, ME9 8AG, UK

    N. Hammatt & R. S. Nelson

  2. Department of Botany, University Park, Nottingham, NG7 2RD, UK

    A. Lister, E. C. Cocking & M. R. Davey

Authors
  1. N. Hammatt
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  2. R. S. Nelson
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  3. A. Lister
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  4. E. C. Cocking
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  5. M. R. Davey
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Editor information

K. J. Puite J. J. M. Dons H. J. Huizing A. J. Kool M. Koornneef F. A. Krens

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© 1988 Kluwer Academic Publishers

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Hammatt, N., Nelson, R.S., Lister, A., Cocking, E.C., Davey, M.R. (1988). Regeneration and Analysis of Callus from Flow-Sorted Heterokaryons of Soybean (Glycine Max) and G. Canescens . In: Puite, K.J., Dons, J.J.M., Huizing, H.J., Kool, A.J., Koornneef, M., Krens, F.A. (eds) Progress in Plant Protoplast Research. Current Plant Science and Biotechnology in Agriculture, vol 7. Springer, Dordrecht. https://doi.org/10.1007/978-94-009-2788-9_90

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  • DOI: https://doi.org/10.1007/978-94-009-2788-9_90

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-94-010-7754-5

  • Online ISBN: 978-94-009-2788-9

  • eBook Packages: Springer Book Archive

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