Abstract
This chapter focuses on methods for epitope mapping on novel viral polypeptrdes and on fine tuning sensitivity and spectficity of the identified peptide antigens for application in virus diagnosis. Because of the development of efficient methods of multiple peptrde synthesis (1–3), antigenic sates of many proteins could be detected
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
References
Jung G and Beck-Sickmger A. G (1992) Multiple peptide synthesis methods and then applications Angew Chem Int Ed Engl 31, 367–486
Beck-Sickinger A G. and Jung G (1993) Epitope mapping synthetic approaches to the understanding of molecular recognition in the immune system Pharmacol Acta Helv. 68, 3–20
Gombert F O, Werz W, Schluter M., Bayer A., Werner R G., Berthold W, and Jung G (1994) Pentapeptide identified as a monoclonal antibody binding site in the serine-protease domain of t-PA Biol Chem Hoppe-Seyler 375, 471–480
Ihlenfeldt H-G, Seidel C, Wienhues U, and Jung G. (1997) Complete Epitope Fine-Mapping of the Hepatitis C Virus-Polyprotein Manuscript submitted
Ihlenfeldt H G (1995) Die B-und T-Zellepitope des Hepatitis C Virus PhD Thesis, University of Tubingen, Germany.
Setdel C, Ehrlich-Wemretch G, Bayer H, Wienhues U, Jung G, and Ihlenfeldt H G (1991) HCV peptide-antigens and a method of testing for the hepatitis C W-US (HCV) Patent Appl DE 9l-4122160.
Seidel C, Wienhues U, Bayer H., Jung G., and Ihlenfeldt H G (1992) HCV peptide-antigens and a method of testing for the hepatitis C virus (HCV) Patent Appl DE 92-4240980
Geysen H M, Meloen R H, and Barteling S J (1984) Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid. Proc Natl. Acad Sci USA 81, 3998–4002.
Wrenhues U, Faatz E, Melchior W, and Bayer H (1993) Boehringer Mannhelm modular test concepts in HIV and hepatitis immunoassays Clin Btochem 26, 295–299
Kraas W, Ihlenfeldt H. G, Seidel C., Wienhues U., and Jung G (1994) Missing of continuous epitopes of HCV by scanning the entire polyprotein with the pin technology, in Abstracts 2nd International meeting on hepatltls C and related viruses. University of California, San Diego, CA, pp. 258.
Kraas W., Ihlenfeldt H. G., Seidel C, Wienhues U., Schmitt U., and Jung G (1995) Comparison of pin technology and biotinylated peptides for epitope mapping and application of identified epitopes in the diagnostic of hepatitis C, in Peptides 1994 (Maia H. L. S., ed), Escom Leaden, The Netherlands, pp. 827,828
Rodda S (1991) Difficult sera partially resolved with biotinylated peptides Pinnacles 1, 10,11.
Wiesmuller K.-H., Treffer U., Spohn R,, and Jung G. (1992) Fully automated synthesis of peptides on polyethylene pins, in Peptides (Schneider C H and Eberle A. N., eds.), Escom, Leiden, The Netherlands, pp. 308,309
Gombert F O, Schaude R., Troger W, Jung G, Rubsamen-Waigmann H, Blecha W., Pfeifer S., Krohn K., Tahtinen M., Ovod V., Ranki A., Shi L., and Wernet P. (1991) Complete epitope fine-mapping of HIV-1 Nef-protein, in Peptides (Giralt E. and Andreu D, eds.), Escom, Leiden, The Netherlands, pp. 872–875.
Choo Q. L., Richman K H., Han J. H., Berger K, Lee C., Dong C, Gallegos C, Colt D., Medina-Selby A, Barr P J., Weiner A J, Bradley D. W., Kuo G, and Houghton M. (1991) Genetic organization and diversity of the hepatitis C virus Proc NatL Acad Sci USA 88, 2451–2455.
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1998 Humana Press Inc.
About this protocol
Cite this protocol
Wienhues, U., Kraas, W., Seidel, C., Ihlenfeidt, H.G., Jung, G. (1998). Mapping of Linear B-Cell Epitopes on Viral Polypeptides by Multiple Peptide Synthesis and Fine Tuning Sensitivity and Specificity of the Identified Peptide Antigens for Application in Virus Diagnosis. In: Reischl, U. (eds) Molecular Diagnosis of Infectious Diseases. Methods in Molecular Medicine™, vol 13. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-485-2:125
Download citation
DOI: https://doi.org/10.1385/0-89603-485-2:125
Publisher Name: Humana Press, Totowa, NJ
Print ISBN: 978-0-89603-485-3
Online ISBN: 978-1-59259-597-6
eBook Packages: Springer Protocols