Skip to main content

Mapping of Linear B-Cell Epitopes on Viral Polypeptides by Multiple Peptide Synthesis and Fine Tuning Sensitivity and Specificity of the Identified Peptide Antigens for Application in Virus Diagnosis

  • Protocol
Molecular Diagnosis of Infectious Diseases

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 13))

  • 4873 Accesses

Abstract

This chapter focuses on methods for epitope mapping on novel viral polypeptrdes and on fine tuning sensitivity and spectficity of the identified peptide antigens for application in virus diagnosis. Because of the development of efficient methods of multiple peptrde synthesis (13), antigenic sates of many proteins could be detected

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Protocol
USD 49.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 89.00
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

References

  1. Jung G and Beck-Sickmger A. G (1992) Multiple peptide synthesis methods and then applications Angew Chem Int Ed Engl 31, 367–486

    Article  Google Scholar 

  2. Beck-Sickinger A G. and Jung G (1993) Epitope mapping synthetic approaches to the understanding of molecular recognition in the immune system Pharmacol Acta Helv. 68, 3–20

    Article  CAS  Google Scholar 

  3. Gombert F O, Werz W, Schluter M., Bayer A., Werner R G., Berthold W, and Jung G (1994) Pentapeptide identified as a monoclonal antibody binding site in the serine-protease domain of t-PA Biol Chem Hoppe-Seyler 375, 471–480

    Article  PubMed  CAS  Google Scholar 

  4. Ihlenfeldt H-G, Seidel C, Wienhues U, and Jung G. (1997) Complete Epitope Fine-Mapping of the Hepatitis C Virus-Polyprotein Manuscript submitted

    Google Scholar 

  5. Ihlenfeldt H G (1995) Die B-und T-Zellepitope des Hepatitis C Virus PhD Thesis, University of Tubingen, Germany.

    Google Scholar 

  6. Setdel C, Ehrlich-Wemretch G, Bayer H, Wienhues U, Jung G, and Ihlenfeldt H G (1991) HCV peptide-antigens and a method of testing for the hepatitis C W-US (HCV) Patent Appl DE 9l-4122160.

    Google Scholar 

  7. Seidel C, Wienhues U, Bayer H., Jung G., and Ihlenfeldt H G (1992) HCV peptide-antigens and a method of testing for the hepatitis C virus (HCV) Patent Appl DE 92-4240980

    Google Scholar 

  8. Geysen H M, Meloen R H, and Barteling S J (1984) Use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid. Proc Natl. Acad Sci USA 81, 3998–4002.

    Article  PubMed  CAS  Google Scholar 

  9. Wrenhues U, Faatz E, Melchior W, and Bayer H (1993) Boehringer Mannhelm modular test concepts in HIV and hepatitis immunoassays Clin Btochem 26, 295–299

    Article  Google Scholar 

  10. Kraas W, Ihlenfeldt H. G, Seidel C., Wienhues U., and Jung G (1994) Missing of continuous epitopes of HCV by scanning the entire polyprotein with the pin technology, in Abstracts 2nd International meeting on hepatltls C and related viruses. University of California, San Diego, CA, pp. 258.

    Google Scholar 

  11. Kraas W., Ihlenfeldt H. G., Seidel C, Wienhues U., Schmitt U., and Jung G (1995) Comparison of pin technology and biotinylated peptides for epitope mapping and application of identified epitopes in the diagnostic of hepatitis C, in Peptides 1994 (Maia H. L. S., ed), Escom Leaden, The Netherlands, pp. 827,828

    Chapter  Google Scholar 

  12. Rodda S (1991) Difficult sera partially resolved with biotinylated peptides Pinnacles 1, 10,11.

    Google Scholar 

  13. Wiesmuller K.-H., Treffer U., Spohn R,, and Jung G. (1992) Fully automated synthesis of peptides on polyethylene pins, in Peptides (Schneider C H and Eberle A. N., eds.), Escom, Leiden, The Netherlands, pp. 308,309

    Google Scholar 

  14. Gombert F O, Schaude R., Troger W, Jung G, Rubsamen-Waigmann H, Blecha W., Pfeifer S., Krohn K., Tahtinen M., Ovod V., Ranki A., Shi L., and Wernet P. (1991) Complete epitope fine-mapping of HIV-1 Nef-protein, in Peptides (Giralt E. and Andreu D, eds.), Escom, Leiden, The Netherlands, pp. 872–875.

    Google Scholar 

  15. Choo Q. L., Richman K H., Han J. H., Berger K, Lee C., Dong C, Gallegos C, Colt D., Medina-Selby A, Barr P J., Weiner A J, Bradley D. W., Kuo G, and Houghton M. (1991) Genetic organization and diversity of the hepatitis C virus Proc NatL Acad Sci USA 88, 2451–2455.

    Article  PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1998 Humana Press Inc.

About this protocol

Cite this protocol

Wienhues, U., Kraas, W., Seidel, C., Ihlenfeidt, H.G., Jung, G. (1998). Mapping of Linear B-Cell Epitopes on Viral Polypeptides by Multiple Peptide Synthesis and Fine Tuning Sensitivity and Specificity of the Identified Peptide Antigens for Application in Virus Diagnosis. In: Reischl, U. (eds) Molecular Diagnosis of Infectious Diseases. Methods in Molecular Medicine™, vol 13. Humana Press, Totowa, NJ. https://doi.org/10.1385/0-89603-485-2:125

Download citation

  • DOI: https://doi.org/10.1385/0-89603-485-2:125

  • Publisher Name: Humana Press, Totowa, NJ

  • Print ISBN: 978-0-89603-485-3

  • Online ISBN: 978-1-59259-597-6

  • eBook Packages: Springer Protocols

Publish with us

Policies and ethics