Overview
- Editors:
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Udo Reischl
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University of Regensburg, Germany
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About this book
In most societies the medical field is undergoing a dramatic reorien- tion. Fundamentally new technologies in diagnosis and therapy, as well as the extension of life expectations, have increased health costs to an extent that now nears the limits of acceptability. One consequence has been a reevaluation of the need for and duration of each individual hospitalization. For diagnostic laboratories, results must now be obtained rapidly in order to include them in therapeutic decisions. Furth- more, therapeutic approaches to the control of infectious agents, limited by the number of therapeutic compounds available and by the growing perce- age of multiresistant agents, now require improvement in the precision of diagnostic approaches. The necessity for maximal reduction of the risk of infection by the development of appropriate pharmaceutical products demands even higher levels of sensitivity in diagnostic tests. Today's highly purified antigens---often from recombinantly generated antigens or peptides, monoclonal or recombinant antibodies, and more sen- tive signal-production and signal-detection systems--have greatly advanced immunologically based detection methodology. However, this approach has biological limits that further enhancement of sensitivity cannot breach. Fortunately nucleic acids have now been quite successfully added to the sp- trum of diagnostic targets. Recombinant-produced antigens and the integration of microelectronics and microfluidics offer new perspectives and possibilities.
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Article
Open access
30 October 2021
Table of contents (36 protocols)
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Recombinant Proteins and Peptide
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Identification of Important Antigens
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- Peng Liang, Feilan Wang, Weimin Zhu, Robert P. O’Connell, Lidia Averboukh
Pages 3-13
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- Kari Johansen, Lennart Svensson
Pages 15-28
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Identification of Corresponding Open Reading Frames
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- Yue Zhang, Michael A. Frohman
Pages 81-105
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Identification of Important Antigenic Epitopes
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- Robert C. Mierendorf, Beth Hammer, Robert E. Novy
Pages 107-123
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- Ulla Wienhues, Wolfgang Kraas, Christoph Seidel, Hans Georg Ihlenfeidt, Günther Jung
Pages 125-142
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Cloning and Expression of Open Reading Frames in Prokaryontic Systems
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- Deborah A. Polayes, T. Dawn Parks, Stephen A. Johnston, William G. Dougherty
Pages 169-183
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Cloning and Expression of Open Reading Frames in Eukaryontic Systems
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- Alan Warnes, Anthony R. Fooks, John R. Stephenson
Pages 201-211
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- Valentina C. Ciccarone, Deborah A. Polayes, Verne A. Luckow
Pages 213-235
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- Valentina C. Ciccarone, Joel A. Jessee, Peter Liljeström
Pages 237-255
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Purification of Recombinant Proteins
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- Robert C. Mierendorf, Barbara B. Morris, Beth Hammer, Robert E. Novy
Pages 257-292
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- Richard E. Kneusel, Joanne Crowe, Melanie Wulbeck, Joachim Ribbe
Pages 293-308
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- Richard E. Kneusel, Melanie Wulbeck, Joachim Ribbe
Pages 309-322
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Reviews
Review of the first edition:
"This work represents one of the most up-to-date molecular biology protocols books to date. . .Anyone who has the interest and resources to develop novel assays not only for infectious diseases but also for other diseases will benefit tremendously from this book. . a valuable purchase to keep as a quick protocol source."-Archives of Pathology and Laboratory Medicine
Editors and Affiliations
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University of Regensburg, Germany
Udo Reischl