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Chiral High Performance Liquid Chromatography of Neutral Glycerolipids

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Encyclopedia of Lipidomics

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Synonyms

Chiral-phase HPLC; Fats and oils; Glycerolipids; Neutral lipids

Introduction

Glycerolipids (neutral acyl- and alkylglycerols, glycerophospholipids, glyceroglycolipids, etc.) are complex mixtures of different types of molecular species that contain stereoisomers. Although technology of chromatography has advanced rapidly in recent years, there are still many lipid species that are difficult to separate and for which specific biological activity and physiological function remain unknown. Therefore, the development of accurate and concise methods for the analysis of lipids is essential. This has led to the developments of mass spectrometry (MS)-based lipidomics methodologies (Han and Gross 2003). However, MS is unable to differentiate among enantiomers and in many instances among diastereomers. This shortcoming is especially evident in the glycerolipids because of the prochiral nature of glycerol. At present, the resolution of a number of stereoisomeric lipid molecules has...

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Authors and Affiliations

  1. Faculty of Fisheries Sciences, Hokkaido University, 3-1-1 Minato-cho, 041-8611, Hakodate, Hokkaido, Japan

    Y. Itabashi

  2. Banting and Best Department of Medical Research, University of Toronto, 112 College St, M5G 1L6, Toronto, ON, Canada

    A. Kuksis

Authors
  1. Y. Itabashi
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  2. A. Kuksis
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Corresponding authors

Correspondence to Y. Itabashi or A. Kuksis .

Editor information

Editors and Affiliations

  1. Yong Loo Lin School of Medicine Centre for Life Sciences, Singapore, Singapore

    Markus R. Wenk

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Itabashi, Y., Kuksis, A. (2016). Chiral High Performance Liquid Chromatography of Neutral Glycerolipids. In: Wenk, M. (eds) Encyclopedia of Lipidomics. Springer, Dordrecht. https://doi.org/10.1007/978-94-007-7864-1_76-1

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  • DOI: https://doi.org/10.1007/978-94-007-7864-1_76-1

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