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Chemoenzymatic Synthesis of Heparin

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Glycoscience: Biology and Medicine

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Abstract

Chemoenzymatic synthesis is a strategy to produce heparin oligosaccharides with clearly defined structures and properties. Chemical synthesis involving protection and deprotection is combined with enzymatic synthesis. Chemical synthesis is used to construct appropriate acceptor and donor molecules, while enzymatic synthesis is used in extension and modification. Recombinant enzymes are prepared based on heparin’s biosynthetic pathway in animals and capsular polysaccharide pathways in bacteria. Chemoenzymatic synthesis relies on a deep understanding of enzyme specificity and the application of modified unnatural chemically synthesized donors and acceptors to perform target-based synthesis of small- to intermediate-sized heparins.

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Authors and Affiliations

  1. Department of Chemistry and Chemical Biology, Center for Biotechnology and Interdisciplinary Studies, Rensselaer Polytechnic Institute, Troy, NY, 12180, USA

    Demetria M. Dickinson & Robert J. Linhardt

  2. Division of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, University of North Carolina, Chapel Hill, NC, 27599, USA

    Jian Liu

Authors
  1. Demetria M. Dickinson
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  2. Jian Liu
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  3. Robert J. Linhardt
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Corresponding author

Correspondence to Demetria M. Dickinson .

Editor information

Editors and Affiliations

  1. Tokyo Metropolitan Institute of Gerontology, Tokyo Metropolitan Geriatric Hospital, Tokyo, Japan

    Tamao Endo

  2. Department of Biomolecular Systems, Max-Planck-Institute of Colloids and Interfaces, Potsdam, Germany

    Peter H. Seeberger

  3. Dept. Biological Chemistry, Johns Hopkins University School of Medicine, Baltimore, Maryland, USA

    Gerald W. Hart

  4. Academia Sinica, Nankang, Taipei, Taiwan

    Chi-Huey Wong

  5. Systems Glycobiology Group, RIKEN-Max Planck Joint Research Center for Systems Chemical Biology, Wako, Saitama, Japan

    Naoyuki Taniguchi

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© 2014 Springer Japan

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Dickinson, D.M., Liu, J., Linhardt, R.J. (2014). Chemoenzymatic Synthesis of Heparin. In: Endo, T., Seeberger, P., Hart, G., Wong, CH., Taniguchi, N. (eds) Glycoscience: Biology and Medicine. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54836-2_42-1

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  • DOI: https://doi.org/10.1007/978-4-431-54836-2_42-1

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  • Publisher Name: Springer, Tokyo

  • Online ISBN: 978-4-431-54836-2

  • eBook Packages: Springer Reference Biomedicine and Life SciencesReference Module Biomedical and Life Sciences

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