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Aging and Senescence of Skin Cells in Culture

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Textbook of Aging Skin

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Abstract

Studying age-related changes in the physiology, biochemistry, and molecular biology of isolated skin cell populations in culture has greatly expanded the understanding of the fundamental aspects of skin aging. The three main cell types that have been studied extensively with respect to cellular aging in vitro are dermal fibroblasts, epidermal keratinocytes, and melanocytes. Serial subcultivation of normal diploid skin cells can be performed only a limited number of times, and the emerging senescent phenotype can be categorized into structural, physiological, biochemical, and molecular phenotypes, which can be used as biomarkers of cellular aging in vitro. The rate and phenotype of aging are different in different cell types. There are both common features and specific features of aging of skin fibroblasts, keratinocytes, melanocytes, and other cell types. A progressive accumulation of damage in all types of macromolecules is a universal feature of cellular aging in all cell types. A progressive failure of molecular maintenance and repair pathways is the ultimate cause of cellular aging in vitro and in vivo.

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Author information

Authors and Affiliations

  1. Laboratory of Cellular Ageing, Department of Molecular Biology and Genetics, Aarhus University, DK8000, Aarhus C, Denmark

    Suresh I. S. Rattan

Authors
  1. Suresh I. S. Rattan
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Correspondence to Suresh I. S. Rattan .

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Editors and Affiliations

  1. Procter & Gamble Co., Cincinnati, Ohio, USA

    Miranda A. Farage

  2. Procter & Gamble Co., Cincinnati, Ohio, USA

    Kenneth W. Miller

  3. Department Dermatology, University of California, San Francisco School of Medicine, San Francisco, California, USA

    Howard I. Maibach

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© 2015 Springer-Verlag Berlin Heidelberg

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Rattan, S.I.S. (2015). Aging and Senescence of Skin Cells in Culture. In: Farage, M., Miller, K., Maibach, H. (eds) Textbook of Aging Skin. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27814-3_50-2

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  • DOI: https://doi.org/10.1007/978-3-642-27814-3_50-2

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Online ISBN: 978-3-642-27814-3

  • eBook Packages: Springer Reference MedicineReference Module Medicine

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