Introduction
ELLIPS (Harding et al. 1987, 2005) and COVOL (Harding et al. 1997; Jones et al. 1999) are pre-compiled sets of FORTRAN algorithms for the representation of low-resolution molecular shape in solution from hydrodynamic measurements and are available from a Microsoft WINDOWS platform. ELLIPS1,2,3, and 4 are simple to use algorithms for the representation of the overall hydrodynamic shape of proteins in solution in terms of triaxial ellipsoids(three perpendicular semi-axes a>b>c and shape characterized by two axial ratios a/b, b/c) and biaxial ellipsoids or “ellipsoids of revolution” where two of the semi-axes are approximated as equal: a prolate ellipsoid, has semi-axes a,b,b an oblate ellipsoid of semi-axes a,a,b, with a>b in both cases and the asymmetry defined by a the axial ratio a/b. COVOL is a related algorithm for evaluating the thermodynamic nonideality parameter B on the basis of the size, shape, and polyelectrolyte properties of a protein in solution. Both ELLIPS...
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References
Ang S, Kogulanathan J, Morris GA, Kök MS, Shewry PR, Tatham AS, Adams GG, Rowe AJ, Harding SE. Structure and heterogeneity of gliadin: a hydrodynamic evaluation. Eur Biophys J. 2010;39:255–61.
Garcia de la Torre J, Carrasco B, Harding SE. SOLPRO: theory and computer program for the prediction of SOLution PROperties of rigid macromolecules and bioparticles. Eur Biophys J. 1997;25:361–72.
Garcia de la Torre J, Harding SE, Carrasco B. Calculation of NMR relaxation, covolume, and scattering-related properties of bead models using the SOLPRO computer program. Eur Biophys J. 1999;28:119–32.
Harding SE. Modelling the gross conformation of assemblies using hydrodynamics. In: Harding SE, Rowe AJ, editors. Dynamic Properties of Biomolecular Assemblies. Cambridge: Royal Society of Chemistry; 1989. p. 32–56.
Harding SE. On the hydrodynamic analysis of macromolecular conformation. Biophys Chem. 1995;55:69–93.
Harding SE, Cölfen H. Inversion formulae for ellipsoid of revolution macromolecular shape functions. Anal Biochem. 1995;228:131–42.
Harding SE, Rowe AJ. Modelling biological macromolecules in solution III: the Λ - R intersection method for triaxial ellipsoids. Int J Biol Macromol. 1982;4:357–61.
Harding SE, Cölfen H, Horton JC. The ELLIPS suite of macromolecular conformation algorithms. Eur Biophys J. 1987;25:347–60.
Harding SE, Horton JC, Winzor DJ. COVOL: an answer to your thermodynamic non-ideality problems? Biochem Soc Trans. 1997;26:737–40.
Harding SE, Cölfen H, Aziz Z. The ELLIPS suite of whole-body protein conformation algorithms for Microsoft Windows. In: Scott DJ, Harding SE, Rowe AJ, editors. Analytical Ultracentrifugation. Techniques and Methods. Cambridge: Royal Society of Chemistry; 2005. p. 460–83.
Jones S, Thornton JM, Winzor DJ, Harding SE, Horton JC. COVOL: an interactive program for evaluating second virial coefficients from the triaxial shape or dimensions of rigid macromolecules. Biophys J. 1999;76:2432–8.
Lu Y, Harding SE, Michaelsen TE, Longman E, Davis KG, Ortega A, Grossmann JG, Sandlie I, Garcia de la Torre J. Solution conformation of wild type and mutant IgG3 and IgG4 immunoglobulins using Crystallohydrodynamics: possible implications for complement activation. Biophys J. 2007;93:3733–44.
Pavlov GM, Rowe AJ, Harding SE. Conformation zoning of large molecules using the analytical ultracentrifuge. Trends Anal Chem. 1997;16:401–4.
Pörschke D, Obst A. An electric field jump apparatus with ns time resolution forelectrooptical measurements at physiological salt concentrations. Rev Sci Instrum. 1991;62:818–20.
Rallison JM, Harding SE. Excluded volumes for pairs of triaxial ellipsoids at dominant Brownian motion. J Colloid Interface Sci. 1985;103:284–9.
Serdyuk IN, Zaccai N, Zaccai G. Methods in Molecular Biophysics: Structure, Dynamics, Function. Cambridge: Cambridge University Press; 2007.
Taylor WR, Thornton JM, Turnell WG. An ellipsoidal approximation of protein shape. J Mol Graph. 1983;1:30–8.
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Harding, S.E. (2013). Ellips and Covol. In: Roberts, G.C.K. (eds) Encyclopedia of Biophysics. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-16712-6_290
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