Abstract:
Technologies for 3D genome-wide mapping of expression patterns will be increasingly important to understand the brain in health and disease. Here, we describe the use of voxelation to reach these goals. The brain is divided into spatially registered cubes which are subjected to high-throughput expression analysis using methods such as microarrays or real-time PCR. The data can then be used to reconstruct expression images reminiscent of those obtained from biomedical imaging systems. We discuss the insights obtained from voxelation of the mouse brain at a volumetric resolution of 11 μl and 1 μl and the human brain at 1 cm3 and 87 μl. The human and mouse studies also incorporated Alzheimer's and Parkinson's disease specimens, giving a better understanding of the molecular pathology of these disorders. Furthermore, we describe useful analytic approaches to understanding the large datasets resulting from voxelation.
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Abbreviations
- Drd2:
-
dopamine D2 receptor
- Nfl:
-
neurofilament light chain
- qRT-PCR:
-
quantitative real time-PCR
- SVD:
-
singular value decomposition
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Chin, M.H., Smith, D.J. (2009). Cubing the Brain: Mapping Expression Patterns Genome-Wide. In: Lajtha, A., Banik, N., Ray, S.K. (eds) Handbook of Neurochemistry and Molecular Neurobiology. Springer, Boston, MA. https://doi.org/10.1007/978-0-387-30375-8_29
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DOI: https://doi.org/10.1007/978-0-387-30375-8_29
Publisher Name: Springer, Boston, MA
Print ISBN: 978-0-387-30343-7
Online ISBN: 978-0-387-30375-8
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