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Safety Pharmacology of Drugs with Osteoarthritis-Related Activity

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Drug Discovery and Evaluation

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1 I.L.1. Cartilage Matrix Turnover in vitro

1.1 I.L.1.1. Modulation of Chondrocytic Proteoglycan Metabolism

1.1.1 Purpose and Rationale

Several agents and therapeutic principles addressing musculoskeletal disorders, applied either systemically or intraarticularly, have been discussed to potentially impair the integrity of articular cartilage (Raynauld et al. 2003; Adams et al. 2000; Theiler et al. 1994). Therefore, a reliable cellular assay is of interest to detect chondrodeleterious properties of a test drug in its early developmental stages.

In primary cultures, articular chondrocytes chondrocytesgrown in an artificial matrix after digestion of the original one, maintain their characteristic synthesis and turnover rate of cartilage matrix macromolecules for a long time. These metabolic processes can be influenced pharmacologically. In the following assay, compounds are tested for their effect upon the normal turnover of cartilage matrix by chondrocytes. The test is used to detect...

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Authors and Affiliations

  1. Fürstenberger Str. 1, 60322, Frankfurt, Germany

    Ruth Raiss

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  1. Ruth Raiss
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Editors and Affiliations

  1. Johann-Wolfgang-Goethe-Universität, Frankfurt am Main, Germany

    H. Gerhard Vogel Prof.

  2. Altstadt 19, 64807, Dieburg, Germany

    Franz Jakob Hock Dr. (Editor) (Editor)

  3. Aventis Pharma Deutschland GmbH, Industriepark Hoechst, 65926, Frankfurt, Germany

    Jochen Maas Dr. (Co-Editor) (Co-Editor)

  4. Alte Poststraße 10, 65510, Idstein, Germany

    Dieter Mayer Prof. (Co-Editor) (Co-Editor)

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Raiss, R. (2006). Safety Pharmacology of Drugs with Osteoarthritis-Related Activity. In: Vogel, H.G., Hock, F.J., Maas, J., Mayer, D. (eds) Drug Discovery and Evaluation. Springer, Berlin, Heidelberg. https://doi.org/10.1007/3-540-29804-5_12

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