Abstract
Novel marker antibodies to human iPS/ES cells that are capable of distinguishing between human embryonal carcinoma (hEC) cells and human embryonic stem (hES) cells were developed. One of these antibodies, R-10G recognizes a type of keratan sulfate lacking oversulfated structures on hiPS/ES cells. R-10G is different from traditional hiPS/ES marker antibodies, TRA-1-60 and TRA-1-81, in its cellular localization and therefore its epitope structure. Thus, R-10G should be useful not only as a new molecular probe for disclosing the roles of glycans on the surface of hiPS cells in the maintenance of self-renewal and pluripotency and during the process of differentiation but also as a potent tool for the evaluation and standardization of hiPS cells of different tissue origins and histories in regenerative medicine.
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Kawasaki, T., Kawasaki, N., Nakao, H., Toyoda, H. (2015). Novel Antibody for Keratan Sulfate Expressed on Human iPS/ES Cells. In: Taniguchi, N., Endo, T., Hart, G., Seeberger, P., Wong, CH. (eds) Glycoscience: Biology and Medicine. Springer, Tokyo. https://doi.org/10.1007/978-4-431-54841-6_94
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DOI: https://doi.org/10.1007/978-4-431-54841-6_94
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Publisher Name: Springer, Tokyo
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Online ISBN: 978-4-431-54841-6
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