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Use of Self-Quenched, Fluorogenic LUX™ Primers for Gene Expression Profiling

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Fluorescent Energy Transfer Nucleic Acid Probes

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 335))

Abstract

Application of a real-time detection system based on a novel primer design in gene expression profiling is described. In this system, called LUXTM (Light Upon eXtension), the generation of signal is based on a single fluorescent dye molecule that is attached to an oligonucleotide close to the 3′-end. A primer design software is available that identifies LUX primer pairs based on a set of rules for optimum signal development. The use of LUX fluorogenic primers to determine the expression patterns of various transcripts during differentiation in the P-19 mouse neuronal model is described.

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Authors
  1. Wolfgang Kusser
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Editors and Affiliations

  1. Baylor College of Medicine, Houston, TX

    Vladimir V. Didenko MD, PhD

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© 2006 Humana Press Inc., Totowa, NJ

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Kusser, W. (2006). Use of Self-Quenched, Fluorogenic LUX™ Primers for Gene Expression Profiling. In: Didenko, V.V. (eds) Fluorescent Energy Transfer Nucleic Acid Probes. Methods in Molecular Biology™, vol 335. Humana Press. https://doi.org/10.1385/1-59745-069-3:115

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  • DOI: https://doi.org/10.1385/1-59745-069-3:115

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-380-0

  • Online ISBN: 978-1-59745-069-0

  • eBook Packages: Springer Protocols

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