Abstract
Multiple myeloma is a plasma cell disorder characterized at the cytogenetic level by aneuploid karyotypes with numerous complex structural aberrations. Unfortunately, conventional chromosome-banding techniques are unable to resolve many of these aberrations. Multicolor Spectral Karyotyping (SKY) is a molecular cytogenetic technique that utilizes chromosomepainting probes for characterizing complex structural chromosome aberrations. This innovative technology allows the simultaneous display of each of the 24 different chromosomes in a different color. Chromosomal sites of rearrangement or translocation between different chromosomes are visualized by a change in the color at the point of the aberration. The SKY technique makes possible the identification of chromosomal bands of unknown origin, including translocations, insertions, complex rearrangements, and marker chromosomes, which, in many cases, go unresolved by traditional banding methods.
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References
Schrƶck E., du Manoir T., Veldman T., et al. (1996) Multicolor spectral kary-otyping of human chromosomes. Science 273, 494ā497.
Rao R H., Cigudos J., Ning Y, et al. (1998) Karyotypic complexity of multiple myeloma defined by multicolour spectral karyotyping (SKY). Blood 92, 1743ā1748.
Sawyer J. R., Lukacs J. L., Munshi N., et al. (1998) Identification of new non-random translocations in multiple myeloma with multicolor spectral karyoytping. Blood 92, 4269ā4278.
Sawyer J. R., Lukacs J. L., Thomas E. L., et al. (2001) Multicolor spectral karyotyping identifies new translocations and a recurring pathway for chromosome instability in multiple myeloma. Br. J. Haematol. 112, 167ā174.
Ho P. J., Campell L. J., Gibson J., Brown R., and Joshua D. (2002) The biology and cytogenetics of multiple myeloma. Rev. Clin. Exp. Hematol. 6, 276ā300.
Bayani J. M. and Squire J. A. (2002) Applications of SKY in cancer cyto-genetics. Cancer Invest. 20, 373ā386.
Garini Y., Macville M., du Manoir S., et al. (1996) Spectral karyotyping. Bioimaging 4, 65ā72.
Telenius H., Pelear A. H., Tunnacliffe A., et al. (1992) Cytogentic analysis by chromosome painting using DOP-PCR amplified flow-sorted chromosomes. Genes Chromosomes Cancer 4, 257ā263.
Hilgenfeld E., Mantagna C., Padilla-Nash H., Stapleton L., Heselmeyer-Haddad K., and Reid T. (2002) Spectral karyotyping in cancer cytogenetics. Methods Mol. Med. 68, 29ā44.
Sawyer J. R., Waldron J. A., Jagannath S., and Barlogie B. (1995) Cytogenetic findings in 200 patients with multiple myeloma. Cancer Genet. Cytogenet. 82, 41ā49.
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Ā© 2005 Humana Press Inc.
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Sawyer, J.R. (2005). Multicolor Spectral Karyotyping in Multiple Myeloma. In: Brown, R.D., Ho, P.J. (eds) Multiple Myeloma. Methods in Molecular Medicineā¢, vol 113. Humana Press. https://doi.org/10.1385/1-59259-916-8:49
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DOI: https://doi.org/10.1385/1-59259-916-8:49
Publisher Name: Humana Press
Print ISBN: 978-1-58829-392-3
Online ISBN: 978-1-59259-916-5
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