Abstract
This chapter provides a manual for a laboratory-based short course to introduce the common techniques of solid-phase peptide synthesis (SPPS). The course provides students the opportunity to design and manually synthesize analogs of glutathione using relatively simple equipment available in any unsophisticated laboratory. The manual provides compact protocols for both the different steps of SPPS and the final cleavage of peptides from resin supports. We also introduce a simple method for the synthesis of combinatorial libraries of glutathione analogs that is suitable for those relatively unfamiliar with the field of peptide chemistry.
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© 2005 Humana Press Inc., Totowa, NJ
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Soomets, U., Zilmer, M., Langel, l. (2005). Manual Solid Phase Synthesis of Glutathione Analogs. In: Howl, J. (eds) Peptide Synthesis and Applications. Methods in Molecular Biology™, vol 298. Humana Press. https://doi.org/10.1385/1-59259-877-3:241
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DOI: https://doi.org/10.1385/1-59259-877-3:241
Publisher Name: Humana Press
Print ISBN: 978-1-58829-317-6
Online ISBN: 978-1-59259-877-9
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