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Quenched Fluorescent Substrate- Based Peptidase Assays

  • Protocol
Peptide Synthesis and Applications

Part of the book series: Methods in Molecular Biology™ ((MIMB,volume 298))

  • 1998 Accesses

Abstract

The use of specific quenched fluorescent substrates (QFS) provides a rapid and sensitive method to measure peptidase activity, and is readily adaptable to highthroughput screening of potential peptidase inhibitors. In this chapter, we discuss general considerations for the development of QFS assays, and describe in detail an assay protocol for the mammalian metallopeptidase, endothelin-converting enzyme.

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References

  1. Johnson, G. D. and Ahn, K. (2000) Development of an internally quenched fluorescent substrate selective for endothelin-converting enzyme-1. Anal. Biochem. 286, 112–118.

    Article  PubMed  CAS  Google Scholar 

  2. Okada, K., Arai, Y., Hata, M., Matsuyama, K., and Yano, M. (1993) Big endothelin-1 structure important for specific processing by endothelin-converting enzyme of bovine endothelial cells. Eur. J. Biochem. 218, 493–498.

    Article  PubMed  CAS  Google Scholar 

  3. Claing, A., Neugebauer, W., Yano, M., Rae, G. A., and D%Orleans-Juste, P. (1995) [Phe22]-big endothelin-1[19-37]: a new and potent inhibitor of the endothelin-converting enzyme. J. Cardiovasc. Pharmacol. 26(Suppl 3), S72–S74.

    PubMed  CAS  Google Scholar 

  4. Keller, P. M., Lee, C. P., Fenwick, A. E., Atkinson, S. T., Elliott, J. D., and DeWolf, W. E. Jr. (1996) Endothelin-converting enzyme: substrate specificity and inhibition by novel analogs of phosphoramidon. Biochem. Biophys. Res. Commun. 223, 372–378.

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Author information

Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia

    Rebecca A. Lew

  2. Department of Pharmaceutical Biology and Pharmacology, Monash University, Parkville, Victoria, Australia

    Nathalie Tochon-Danguy

  3. Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria, Australia

    Catherine A. Hamilton, Karen M. Stewart, Marie-Isabel Aguilar & A. Ian Smith

Authors
  1. Rebecca A. Lew
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  2. Nathalie Tochon-Danguy
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  3. Catherine A. Hamilton
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  4. Karen M. Stewart
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  5. Marie-Isabel Aguilar
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  6. A. Ian Smith
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Editor information

Editors and Affiliations

  1. Research Institute in Healthcare Science, School of Applied Sciences, University of Wolverhampton, Wolverhampton, UK

    John Howl

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© 2005 Humana Press Inc., Totowa, NJ

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Lew, R.A., Tochon-Danguy, N., Hamilton, C.A., Stewart, K.M., Aguilar, MI., Smith, A.I. (2005). Quenched Fluorescent Substrate- Based Peptidase Assays. In: Howl, J. (eds) Peptide Synthesis and Applications. Methods in Molecular Biology™, vol 298. Humana Press. https://doi.org/10.1385/1-59259-877-3:143

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  • DOI: https://doi.org/10.1385/1-59259-877-3:143

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-317-6

  • Online ISBN: 978-1-59259-877-9

  • eBook Packages: Springer Protocols

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