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Designing TCR for Cancer Immunotherapy

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Adoptive Immunotherapy: Methods and Protocols

Part of the book series: Methods in Molecular Medicine™ ((MIMM,volume 109))

Abstract

Reprogramming T-cell populations by T-cell receptor (TCR) gene transfer is a new therapeutic tool for adoptive tumor immunotherapy. Gene transfer of human leukocyte antigen (HLA)-transgenic mice-derived TCR into human T-cells allows the circumvention of tolerance to tumor-associated (self) antigens (TAA). This chapter reports on the identification of the α and β chains of the heterodimeric TCR derived from a mouse T-cell clone. The related DNA fragments are inserted into a retroviral vector for heterologous expression of the TAA-specific TCR in human T-cells. Polymerase chain reaction (PCR)-based cloning protocols are provided for the tailor-made customization of murine TCR. We describe the humanization and chimerization of such TCR as well as their expression in human T-cells.

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Author information

Authors and Affiliations

  1. Department of Hematology and Oncology, Johannes Gutenberg-University, Mainz, Germany

    Ralf-Holger Voss

  2. Department of Hematology and Oncology, Johannes Gutenberg-University Mainz, Mainz, Germany

    Jürgen Kuball

  3. Department of Hematology and Oncology, Johannes Gutenberg-University, Mainz, Germany

    Matthias Theobald

Authors
  1. Ralf-Holger Voss
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  2. Jürgen Kuball
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  3. Matthias Theobald
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Editor information

Editors and Affiliations

  1. Research Department, Kantonsspital St. Gallen, Switzerland

    Burkhard Ludewig

  2. Department of Abdominal and Transplant Surgery, Hannover Medical School, Hannover, Germany

    Matthias W. Hoffmann

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© 2005 Humana Press Inc.

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Voss, RH., Kuball, J., Theobald, M. (2005). Designing TCR for Cancer Immunotherapy. In: Ludewig, B., Hoffmann, M.W. (eds) Adoptive Immunotherapy: Methods and Protocols. Methods in Molecular Medicine™, vol 109. Humana Press. https://doi.org/10.1385/1-59259-862-5:229

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  • DOI: https://doi.org/10.1385/1-59259-862-5:229

  • Publisher Name: Humana Press

  • Print ISBN: 978-1-58829-406-7

  • Online ISBN: 978-1-59259-862-5

  • eBook Packages: Springer Protocols

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