Abstract
Activated platelets expose P-selectin and thereby adhere to leukocytes of all main classes (1–4). If neutrophils or monocytes are also activated, then adhesion can be stabilized by binding of their β2-integrins to fibrinogen and possibly other ligands on the platelet surface (5–9). Experimentally, adhesion can be demonstrated when leukocytes are flowed over immobilized platelets (3,4,6–8), or when suspensions of isolated leukocytes and platelets are mixed together (1,5). Platelet-leukocyte aggregates can also be found in whole blood (10,11). Mutually activating interactions can also occur between leukocytes and platelets through released substances, and these interactions are promoted by adhesion between the cells (12). Platelet-leukocyte adhesion may thus be influential in the development of inflammation or of thrombi. Platelets are not thought to bind to intact endothelium normally, but increasing evidence suggests that adhesion can occur when endothelial cells are stimulated or exposed to pathogenic agents (e.g., 13–15). Among other actions, platelets attached to endothelium might assist capture of flowing leukocytes (16).
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References
Hamburger, S. A. and McEver, R. P. (1990) GMP-140 mediates adhesion of stimulated platelets to neutrophils. Blood 75, 550–554.
Moore, K. L., Stults, N. L., Diaz, S., Smith, D. F., Cummings, R. D., Varki, A., et al. (1992) Identification of a specific glycoprotein ligand for P-selectin (CD62) on myeloid cells. J. Cell Biol. 118, 445–456.
Lalor, P. and Nash, G. B. (1995) Adhesion of flowing leucocytes to immobilized platelets. Br. J. Haematol. 89, 725–732.
Nash, G. B., Morland, C., Sheikh, S., Buttrum, S. M., and Lalor, P. (1996) Adhesion between leucocytes and platelets, rheology, mechanisms and consequences. Prog. Appl. Microcirc. 22, 98–113.
Spangenberg, P., Redlich, H., Bergmann, I., Losche, W., Gotzrath, M., and Kehrel, B. (1993) The platelet glycoprotein IIb/IIIa complex is involved in the adhesion of activated platelets to leukocytes. Thrombo. Haemost. 70, 514–521.
Diacovo, T. G., deFougerolles, A. R., Bainton, D. F., and Springer, T. A. (1994) A functional integrin ligand on the surface of platelets, intercellular adhesion molecule-2. J. Clin. Invest. 94, 1243–1251.
Sheikh, S. and Nash, G. B. (1996) Continuous activation and deactivation of integrin CD11b/CD18 during de novo expression enables rolling neutrophils to immobilize on platelets. Blood 87, 5040–5050.
Weber, C. and Springer, T. A. (1997) Neutrophil accumulation on activated, surface-adherent platelets in flow is mediated by interaction of Mac-1 with fibrinogen bound to αIIbβ3 and stimulated by platelet-activating factor. J. Clin. Invest. 100, 2085–2093.
Kuijper, P. H. M., Torres, H. I. G., Lammers, J. W. J., Sixma, J. J., Koenderman, L., and Zwaginga, J. J. (1998) Platelet associated fibrinogen and ICAM-2 induce firm adhesion of neutrophils under flow. Thromb. Haemost. 80, 443–448.
Rinder, C. S., Bonan, J. L., Rinder, H. M, Ault, K. A., and Smith B. R. (1991) Dynamics of leukocyte-platelet adhesion in whole blood. Blood 78, 1730–1737.
Rinder, C. S, Bonan, J. L., Rinder, H. M., Mathew, J., Hines, R., and Smith B. R. (1992) Cardiopulmonary bypass induces leukocyte-platelet adhesion. Blood 79, 1201–1205.
Evangelista, V., Piccardoni, P., White, J. G., deGaetano, G., and Cerletti, C. (1993) Cathepsin G-dependent platlet stimulation by activated polymorphonuclear leukocytes and its inhibition by antiproteinases, role of P-selectin mediated cell-cell adhesion. Blood 70, 2947–2957.
Rosenblum, W. I. (1997) Platelet adhesion and aggregation without endothelial denudation or exposure of basal lamina and/or collagen. J. Vasc. Res. 34, 409–417.
Venturini, C. M., Weston, L. K., and Kaplan, J. E. (1992) Platelet cGMP but not cAMP inhibits thrombin-induced platelet adhesion to pulmonary endothelium. Am. J. Physiol. 263, H606–H612.
Frenette, P. S., Johnson, R. C., Hynes, R. O., and Wagner, D. D. (1992) Platelets roll on stimulated endothelium in vivo, an interaction mediated by endothelial P-selectin. Proc. Nat. Acad. Sci. USA 92, 7450–7454.
Kirton, C. and Nash, G. B. (2000) Activated platelets adherent to an intact endothelial cell monolayer bind flowing neutrophils and enable them to transfer to the endothelial surface. J. Lab. Clin. Med. 136, 303–313.
Stone, P. C. W. and Nash, G. B. (1999) Conditions under which immobilised platelets activate as well as capture flowing neutrophils. Br. J. Haematol. 105, 514–522.
Rainger, G. E., Buckley, C., Simmons, D., and Nash, G. B. (1997) Cross-talk between cell adhesion molecules regulates the migration velocity of neutrophils. Curr. Biol. 7, 316–325.
Buttrum, S. M., Hatton, R., and Nash, G. B. (1993) Selectin-mediated rolling of neutrophils on immobilised platelets. Blood 82, 1165–1174.
Nash, G. B., Abbitt, K. B., Tate, K., Jetha, K. A., and Egginton, S. (2001) Changes in the mechanical and adhesive behaviour of neutrophils on cooling in vitro. Eur. J. Physiol. 442, 762–770.
Yeo, E. L., Sheppard, J. A. I., and Feuerstein, I. A. (1994) Role of P-selectin and leukocyte activation in polymorphonuclear cell adhesion to surface adherent activated platelets under physiologic shear conditions (an injury vessel wall model). Blood 83, 2498–2507.
Ostrovsky, L., King, A. J., Bond, S., Mitchell, D., Lorant, D. E., Zimmerman, G. A., et al. (1998) A juxtacrine mechanism for neutrophil adhesion on platelets involves platelet-activating factor and a selectin-dependent activation process. Blood 91, 3028–3036.
Jungi, T. W., Spycher, M. O., Nydegger, U. E., and Barandun, S. (1986) Platelet-leukocyte interactions, selective binding of thrombin stimulated platelets to human monocytes, polymorphonuclear leukocytes and related cell lines. Blood 67, 629–636.
Maeda, T., Nash, G. B., Christopher, B., Pecsvarady, Z., and Dormandy, J. A. (1991) Platelet-induced granulocyte aggregation in vitro. Blood Coag. Fibrinol. 2, 699–703.
Rhee, B.-G., Hall, E. R., and McIntire, L. V. (1986) Platelet modulation of polymorphonuclear leukocyte shear induced aggregation. Blood 67, 240–246.
Abbitt, K. B. and Nash, G. B. (2001) Characteristics of leukocyte adhesion directly observed in flowing whole blood in vitro. Br. J. Haematol. 112, 55–63.
Kirchhofer, D., Riederer, M. A., and Baumgartner, H. R. (1997) Specific accumulation of circulating monocytes and polymorphonuclear leukocytes on platelet thrombi in a vascular injury model. Blood 89, 1270–1278.
Goldsmith, H. L. and Turitto, V. T. (1986) Rheological aspects of thrombosis and haemostasis, basic principles and applications. Thrombos. Haemost. 55, 415–435.
Tangelder, G. J., Slaaf, D. W., Arts, T., and Reneman, R. S. (1988) Wall shear rate in arterioles in vivo, least estimates from platelet velocity profiles. Am. J. Physiol. 254, H1059–H1064.
Cooke, B. M., Perry, I., Usami, S., and Nash, G. B. (1993) A simplified method for culture of endothelial cells and analysis of adhesion of blood cells under conditions of flow. Microvasc. Res. 45, 33–45.
Kuijper, P. H. M., Torres, H. I. G., vanderLinden, J. A. M., Lammers, J. W. J., Sixma, J. J., Koenderman, L., et al. (1996) Platelet-dependent primary hemostasis promotes selectin and integrin-mediated neutrophil adhesion to damaged endothelium under flow conditions. Blood 87, 3271–3281.
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Nash, G.B. (2004). Adhesion Between Platelets and Leukocytes or Endothelial Cells. In: Gibbins, J.M., Mahaut-Smith, M.P. (eds) Platelets and Megakaryocytes. Methods In Molecular Biology™, vol 272. Humana Press. https://doi.org/10.1385/1-59259-782-3:199
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DOI: https://doi.org/10.1385/1-59259-782-3:199
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