Abstract
Conventional methods for the evaluation of antimicrobials and disinfecting solutions with microorganisms involve culture-based techniques, which are time-consuming and underestimate the number of viable organisms. Rapid detection and viability measurements of microorganisms in homogenous and heterogenous microbial populations have been greatly enhanced by recent advances in the use of fluorescent stains in flow cytometry (FCM) (1–5). FCM has been applied to enumerate, differentiate, and identify microorganisms, determine protein and DNA content of cells, analyze the physiological state of individual cells, and analyze the interaction of drugs, antibiotics, and antimicrobials with microbial cells (1–14). Four physiological states of cells can be distinguished by FCM: (1) reproductively viable, (2) metabolically active, (3) intact, and (4) permeabilized (15).
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© 2004 Humana Press Inc.Totowa, NJ
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Noble-Wang, J.A., Zhang, S., Price, D., Ahearn, D.G. (2004). Viability of Amoebae, Fungal Conidia, and Yeasts. In: Spencer, J.F.T., Ragout de Spencer, A.L. (eds) Public Health Microbiology. Methods in Molecular Biology, vol 268. Humana Press. https://doi.org/10.1385/1-59259-766-1:153
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DOI: https://doi.org/10.1385/1-59259-766-1:153
Publisher Name: Humana Press
Print ISBN: 978-1-58829-117-2
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